ReferenceID 870

Mori Ramulus Inhibits Pancreatic β-Cell Apoptosis and Prevents Insulin Resistance by Restoring Hepatic Mitochondrial Function

Antioxidants (Basel)

Type 2 diabetes mellitus is characterized by insulin resistance and pancreatic beta (beta)-cell dysfunction. Accumulating evidence suggests that mitochondrial dysfunction may cause insulin resistance in peripheral tissue

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Reference Id
870
Evidence Id
17460
Core Evidence Id
17460
Source Reference Id
1736
Herb2 Reference Id
HBREF002533
Subject Paper Key
HERB004837_34204891
Pubmed Id
34204891
Doi
10.3390/antiox10060901
Paper Title
Mori Ramulus Inhibits Pancreatic β-Cell Apoptosis and Prevents Insulin Resistance by Restoring Hepatic Mitochondrial Function
Paper Abstract
Type 2 diabetes mellitus is characterized by insulin resistance and pancreatic beta (beta)-cell dysfunction. Accumulating evidence suggests that mitochondrial dysfunction may cause insulin resistance in peripheral tissues. As commercial hypoglycemic drugs have side effects, it is necessary to develop safe and effective natural compound-based hypoglycemic treatments. This study aimed to investigate the hypoglycemic effects of Mori Ramulus ethanol extract (ME) in a high-fat diet (HFD)-induced diabetes mouse model to decipher the underlying mechanisms focusing on apoptosis and mitochondrial function. ME significantly decreased tunicamycin-induced apoptotic cell death and increased insulin secretion following glucose stimulation in NIT-1 pancreatic beta-cells. Tunicamycin-exposed NIT-1 pancreatic beta-cells showed elevated reactive oxygen species levels and reduced mitochondrial membrane potential, which were reversed by ME treatment. ME inhibited the tunicamycin-induced apoptosis cascade in tunicamycin-exposed NIT-1 pancreatic beta-cells. In HFD diabetic mice, the serum-free fatty acid and insulin levels decreased following a 15-week ME administration. Glucose and insulin tolerance tests showed that ME improved insulin sensitivity. Moreover, ME ameliorated pancreatic beta-cell mass loss in diabetic mice. Finally, ME-treated HFD-fed mice showed improved hepatic mitochondrial function resulting in insulin sensitivity in target tissues. Thus, ME provides protection against pancreatic beta-cell apoptosis and prevents insulin resistance by improving mitochondrial function.
Journal
Antioxidants (Basel)
Publish Year
2021
Experiment Subject
mouse; nit-1 pancreatic beta-cells; tunicamycin-exposed nit-1 pancreatic beta-cells
Experiment Type
Animal Experiment
Phenotype Related
Mitochondrial Dysfunction; Type 2 Diabetes Mellitus; Diabetic; Pancreatic Beta (beta)-cell Dysfunction; Diabetes
Paper Title Cn
Paper Title En
Mori Ramulus Inhibits Pancreatic β-Cell Apoptosis and Prevents Insulin Resistance by Restoring Hepatic Mitochondrial Function
Bilingual Status
semi_complete