ReferenceID 869
Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo
Int J Mol Sci
Mulberry ( Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose
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- Reference Id
- 869
- Evidence Id
- 17459
- Core Evidence Id
- 17459
- Source Reference Id
- 1735
- Herb2 Reference Id
- HBREF002532
- Subject Paper Key
- HERB004834_35887036
- Pubmed Id
- 35887036
- Doi
- 10.3390/ijms23147694
- Paper Title
- Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo
- Paper Abstract
- Mulberry ( Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was to select the MLF with the best anti-inflammatory and antioxidative activities from MLFs eluted by different ethanol concentrations (30%, 50%, and 75%) and explore its pharmacological properties. Three types of MLFs inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in lipopolysaccharide (LPS)-induced RAW 264.7 cells. All MLFs boosted the antioxidative capacity by decreasing the reactive oxygen species (ROS) production and the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and improving the metal ion chelating activity and reducing power. The results revealed that the MLFs eluted by 30% ethanol exhibited the best anti-inflammatory and antioxidative activities. A nontargeted metabolomic analysis was used to analyze 24 types of differential flavonoids between the MLFs. Quercetin, kaempferol, and their derivatives in 30%MLF were more abundant than the other two MLFs. Furthermore, we evaluated the pharmacological activities of 30%MLF in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice. The 30%MLF could alleviate the clinical symptoms, reduce the secretion of inflammatory cytokines, and inhibit the activation of the inflammatory pathway in DSS-induced colitis mice. This study will provide valuable information for the development of MLFs eluted by 30% ethanol as a functional food.
- Journal
- Int J Mol Sci
- Publish Year
- 2022
- Experiment Subject
- mouse; raw 264.7 cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Colitis; Ulcerative Colitis
- Paper Title Cn
- Paper Title En
- Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo
- Bilingual Status
- semi_complete