ReferenceID 4329
Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells
Nutrients
Physiological processes in skin are associated with exposure to UV light and are essential for skin maintenance and regeneration. Here, we investigated whether the leaf and callus extracts of Perilla frutescens (Perilla)
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Record Fields
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- Reference Id
- 4329
- Evidence Id
- 20919
- Core Evidence Id
- 20919
- Source Reference Id
- 1928
- Herb2 Reference Id
- HBREF002725
- Subject Paper Key
- HERB007208_33921322
- Pubmed Id
- 33921322
- Doi
- 10.3390/nu13041263
- Paper Title
- Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells
- Paper Abstract
- Physiological processes in skin are associated with exposure to UV light and are essential for skin maintenance and regeneration. Here, we investigated whether the leaf and callus extracts of Perilla frutescens (Perilla), a well-known Asian herb, affect DNA damage response and repair in skin and keratinocytes exposed to Untraviolet B (UVB) light. First, we examined the protective effects of Perilla leaf extracts in UVB damaged mouse skin in vivo. Second, we cultured calluses using plant tissue culture technology, from Perilla leaf explant and then examined the effects of the leaf and callus extracts of Perilla on UVB exposed keratinocytes. HaCaT cells treated with leaf and callus Perilla extracts exhibited antioxidant activities, smaller DNA fragment tails, and enhanced colony formation after UVB exposure. Interestingly, keratinocytes treated with the leaf and callus extracts of Perilla showed G1/S cell cycle arrest, reduced protein levels of cyclin D1, Cyclin Dependent Kinase 6 (CDK6), and gammaH2AX, and enhanced levels of phosphorylated checkpoint kinase 1 (pCHK1) following UVB exposure. These observations suggest that the leaf and callus extracts of Perilla are candidate nutraceuticals for the prevention of keratinocyte aging.
- Journal
- Nutrients
- Publish Year
- 2021
- Experiment Subject
- mouse; hacat cells; uvb exposed keratinocytes
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells
- Bilingual Status
- semi_complete