ReferenceID 4220
Protective effects of Panax ginseng berry extract on blue light-induced retinal damage in ARPE-19 cells and mouse retina
J Ginseng Res
Background: Age-related macular degeneration (AMD) is a significant visual disease that induces impaired vision and irreversible blindness in the elderly. However, the effects of ginseng berry extract (GBE) on the retina
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- Reference Id
- 4220
- Evidence Id
- 20810
- Core Evidence Id
- 20810
- Source Reference Id
- 1701
- Herb2 Reference Id
- HBREF002498
- Subject Paper Key
- HERB004609_36644394
- Pubmed Id
- 36644394
- Doi
- 10.1016/j.jgr.2022.04.002
- Paper Title
- Protective effects of Panax ginseng berry extract on blue light-induced retinal damage in ARPE-19 cells and mouse retina
- Paper Abstract
- Background: Age-related macular degeneration (AMD) is a significant visual disease that induces impaired vision and irreversible blindness in the elderly. However, the effects of ginseng berry extract (GBE) on the retina have not been studied. Therefore, this study aimed to investigate the protective effects of GBE on blue light (BL)-induced retinal damage and elucidate its underlying mechanisms in human retinal pigment epithelial cells (ARPE-19 cells) and Balb/c retina. Methods: To investigate the effects and underlying mechanisms of GBE on retinal damage in vitro , we performed cell viability assay, pre-and post-treatment of sample, reactive oxygen species (ROS) assay, quantitative real-time PCR (qRT-PCR), and western immunoblotting using A2E-laden ARPE-19 cells with BL exposure. In addition, Balb/c mice were irradiated with BL to induce retinal degeneration and orally administrated with GBE (50, 100, 200 mg/kg). Using the harvested retina, we performed histological analysis (thickness of retinal layers), qRT-PCR, and western immunoblotting to elucidate the effects and mechanisms of GBE against retinal damage in vivo . Results: GBE significantly inhibited BL-induced cell damage in ARPE-19 cells by activating the SIRT1/PGC-1α pathway, regulating NF-kB translocation, caspase 3 activation, PARP cleavage, expressions of apoptosis-related factors (BAX/BCL-2, LC3-Ⅱ, and p62), and ROS production. Furthermore, GBE prevented BL-induced retinal degeneration by restoring the thickness of retinal layers and suppressed inflammation and apoptosis via regulation of NF-kB and SIRT1/PGC-1α pathway, cleavage of caspase 3 and PARP, and expressions of apoptosis-related factors in vivo . Conclusions: GBE could be a potential agent to prevent dry AMD and progression to wet AMD.
- Journal
- J Ginseng Res
- Publish Year
- 2023
- Experiment Subject
- mouse; human; a2e-laden arpe-19 cells; arpe-19 cells; ginseng
- Experiment Type
- Cell Experiment
- Phenotype Related
- Age-related Macular Degeneration; Bl-induced Retinal Degeneration; Impaired Vision; Irreversible Blindness; Visual Disease; Retinal Degeneration
- Paper Title Cn
- Paper Title En
- Protective effects of Panax ginseng berry extract on blue light-induced retinal damage in ARPE-19 cells and mouse retina
- Bilingual Status
- semi_complete