ReferenceID 4007
A polysaccharide extract from the medicinal plant Maidong inhibits the IKK-NF-κB pathway and IL-1β-induced islet inflammation and increases insulin secretion
J Biol Chem
The herb dwarf lilyturf tuber (Maidong, Ophiopogonis Radix) is widely used in Chinese traditional medicine to manage diabetes and its complications. However, the role of Maidong polysaccharide extract (MPE) in pancreatic
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Record Fields
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- Reference Id
- 4007
- Evidence Id
- 20597
- Core Evidence Id
- 20597
- Source Reference Id
- 1296
- Herb2 Reference Id
- HBREF002093
- Subject Paper Key
- HERB001010_32605924
- Pubmed Id
- 32605924
- Doi
- 10.1074/jbc.RA120.014357
- Paper Title
- A polysaccharide extract from the medicinal plant Maidong inhibits the IKK-NF-κB pathway and IL-1β-induced islet inflammation and increases insulin secretion
- Paper Abstract
- The herb dwarf lilyturf tuber (Maidong, Ophiopogonis Radix) is widely used in Chinese traditional medicine to manage diabetes and its complications. However, the role of Maidong polysaccharide extract (MPE) in pancreatic beta-cell function is unclear. Here, we investigated whether MPE protects beta-cell function and studied the underlying mechanisms. We treated db/db and high-fat diet (HFD)-induced obese mice with 800 or 400 mg/kg MPE or water for 4 weeks, followed by an oral glucose tolerance test. Pancreas and blood were collected for molecular analyses, and clonal MIN6 beta-cells and primary islets from HFD-induced obese mice and normal chow diet-fed mice were used in additional analyses. In vivo, MPE both increased insulin secretion and reduced blood glucose in the db/db mice but increased only insulin secretion in the HFD-induced obese mice. MPE substantially increased the beta-cell area in both models (3-fold and 2-fold, p < 0.01, for db/db and HFD mice, respectively). We observed reduced nuclear translocation of the p65 subunit of NF-kappaB in islets of MPE-treated db/db mice, coinciding with enhanced glucose-stimulated insulin secretion (GSIS). In vitro, MPE potentiated GSIS and decreased interleukin 1beta (IL-1beta) secretion in MIN6 beta-cells. Incubation of MIN6 cells with tumor necrosis factor alpha (TNFalpha), interferon-gamma, and IL-1beta amplified IL-1beta secretion and inhibited GSIS. These effects were partially reversed with MPE or the IkappaB kinase beta inhibitor PS1145, coinciding with reduced activation of p65 and p-IkappaB in the NF-kappaB pathway. We conclude that MPE may have potential for therapeutic development for beta-cell protection.
- Journal
- J Biol Chem
- Publish Year
- 2020
- Experiment Subject
- mouse; clonal min6 beta-cells; min6 beta-cells; min6 cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Tumor; Diabetes; Obese
- Paper Title Cn
- Paper Title En
- A polysaccharide extract from the medicinal plant Maidong inhibits the IKK-NF-κB pathway and IL-1β-induced islet inflammation and increases insulin secretion
- Bilingual Status
- semi_complete