ReferenceID 993
Effects of 23-epi-26-deoxyactein on adipogenesis in 3T3-L1 preadipocytes and diet-induced obesity in C57BL/6 mice
Phytomedicine
BACKGROUND: The ethanolic extract of Actaea racemosa L. (Cimicifuga racemosa (L.) Nutt.) has recently been reported to ameliorate obesity-related insulin resistance, hyperlipidemia, and fatty liver in rodents. However, i
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- Reference Id
- 993
- Evidence Id
- 17583
- Core Evidence Id
- 17583
- Source Reference Id
- 1990
- Herb2 Reference Id
- HBREF002787
- Subject Paper Key
- HBIN005002_32570112
- Pubmed Id
- 32570112
- Doi
- 10.1016/j.phymed.2020.153264
- Paper Title
- Effects of 23-epi-26-deoxyactein on adipogenesis in 3T3-L1 preadipocytes and diet-induced obesity in C57BL/6 mice
- Paper Abstract
- BACKGROUND: The ethanolic extract of Actaea racemosa L. (Cimicifuga racemosa (L.) Nutt.) has recently been reported to ameliorate obesity-related insulin resistance, hyperlipidemia, and fatty liver in rodents. However, it remains unclear which A. racemosa components are responsible for these beneficial effects. PURPOSE: We aimed to examine the anti-obesity potential of 23-epi-26-deoxyactein (DA), which is contained in the ethanolic extracts of A. racemosa. STUDY DESIGN AND METHODS: To evaluate the effects of DA on adipogenesis in 3T3-L1 preadipocytes and diet-induced obesity in C57BL/6 mice, in vitro and in vivo tests were performed. For in vitro assessment, we used Oil red O staining that showed lipid accumulation in differentiated 3T3-L1 cells. For in vivo tests, male 5-week-old C57BL/6 mice were fed with low-fat diet (LFD), high-fat diet (HFD), HFD with 10 mg/kg/d luteolin (LU; positive control drug), HFD with 1 mg/kg/d DA, and HFD with 5 mg/kg/d DA for 12 weeks, respectively. Glucose and insulin tolerance tests were performed at week 17. The lipid deposition of adipose tissue and liver was visualized by hematoxylin and eosin staining. Real-time PCR showed mRNA levels of genes involved in adipogenesis, lipogenesis, and lipolysis. AMPK signaling and SIRT1-FOXO1 pathway were assessed by real-time PCR and western blot. RESULTS: 10 muM DA and 20 muM LU treatments inhibited 3T3-L1 adipogenesis through down-regulating the expression of C/ebpalpha, C/ebpbeta, and Ppargamma, which are the critical adipogenic transcription factors. The in vivo results showed that 5 mg/kg/d DA and 10 mg/kg/d LU significantly lowered body weight gain, fat mass, and liver weight in HFD-fed mice. Meanwhile, DA and LU also reduced insulin resistance and serum lipoprotein levels in HFD-fed mice. Mechanistic studies showed that DA and LU promoted adipocyte lipolysis in mice through activating the AMPK signaling and SIRT1-FOXO1 pathway. CONCLUSION: The in vitro results indicate that 10 muM DA suppresses adipogenesis in 3T3-L1 preadipocytes. The in vivo treatment with 5 mg/kg/d DA ameliorates diet-induced obesity in mice, suggesting that DA is a promising natural compound for the treatment of obesity and related metabolic diseases.
- Journal
- Phytomedicine
- Publish Year
- 2020
- Experiment Subject
- mouse; 3t3-l1 preadipocytes; differentiated 3t3-l1 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Obesity; Metabolic Diseases; Hyperlipidemia
- Paper Title Cn
- Paper Title En
- Effects of 23-epi-26-deoxyactein on adipogenesis in 3T3-L1 preadipocytes and diet-induced obesity in C57BL/6 mice
- Bilingual Status
- semi_complete