ReferenceID 981
20(S)-ginsenoside Rg3 promotes myoblast differentiation and protects against myotube atrophy via regulation of the Akt/mTOR/FoxO3 pathway
Biochem Pharmacol
We previously found that 20(S)-ginsenoside Rg3 (S-Rg3) promotes myoblast differentiation via an unknown mechanism. Here we measured levels of myosin heavy chain (MHC) and myogenin, markers of myoblast differentiation, us
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 981
- Evidence Id
- 17571
- Core Evidence Id
- 17571
- Source Reference Id
- 1964
- Herb2 Reference Id
- HBREF002761
- Subject Paper Key
- HBIN003500_32653593
- Pubmed Id
- 32653593
- Doi
- 10.1016/j.bcp.2020.114145
- Paper Title
- 20(S)-ginsenoside Rg3 promotes myoblast differentiation and protects against myotube atrophy via regulation of the Akt/mTOR/FoxO3 pathway
- Paper Abstract
- We previously found that 20(S)-ginsenoside Rg3 (S-Rg3) promotes myoblast differentiation via an unknown mechanism. Here we measured levels of myosin heavy chain (MHC) and myogenin, markers of myoblast differentiation, using Western blot analysis and immunofluorescence staining. Notably, S-Rg3 treatment of C2C12 myoblasts led to increased muscle differentiation and protection from muscle atrophy in a dexamethasone (DEX)-treated C2C12 myotube-based muscle atrophy model. This effect was likely caused by S-Rg3 treatment-induced promotion of Akt/mTOR phosphorylation and inhibition of FoxO3 nuclear transcription. Additionally, S-Rg3 treatment also led to increased fruit fly climbing distances (Drosophila melanogaster) and prevented muscle atrophy in aged fruit flies. Our study provides a mechanistic framework for understanding how S-Rg3 enhances myoblast differentiation and inhibits myotube atrophy through activation of the Akt/mTOR/FoxO3 signaling pathway, as demonstrated in vitro in C2C12 cells and in vivo in fruit flies.
- Journal
- Biochem Pharmacol
- Publish Year
- 2020
- Experiment Subject
- c2c12 cells; c2c12 myoblasts
- Experiment Type
- Cell Experiment
- Phenotype Related
- Myotube Atrophy; Muscle Atrophy
- Paper Title Cn
- Paper Title En
- 20(S)-ginsenoside Rg3 promotes myoblast differentiation and protects against myotube atrophy via regulation of the Akt/mTOR/FoxO3 pathway
- Bilingual Status
- semi_complete