ReferenceID 972
Natural compound catechol induces DNA damage, apoptosis, and G1 cell cycle arrest in breast cancer cells
Phytother Res
Targeting cell cycle and inducing DNA damage by activating cell death pathways are considered as effective therapeutic strategy for combating breast cancer progression. Many of the naturally known small molecules target
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Record Fields
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- Reference Id
- 972
- Evidence Id
- 17562
- Core Evidence Id
- 17562
- Source Reference Id
- 1938
- Herb2 Reference Id
- HBREF002735
- Subject Paper Key
- HBIN000735_33289235
- Pubmed Id
- 33289235
- Doi
- 10.1002/ptr.6970
- Paper Title
- Natural compound catechol induces DNA damage, apoptosis, and G1 cell cycle arrest in breast cancer cells
- Paper Abstract
- Targeting cell cycle and inducing DNA damage by activating cell death pathways are considered as effective therapeutic strategy for combating breast cancer progression. Many of the naturally known small molecules target these signaling pathways and are effective against resistant and/or aggressive types of breast cancers. Here, we investigated the effect of catechol, a naturally occurring plant compound, for its specificity and chemotherapeutic efficacies in breast cancer (MCF-7 and MDA-MB-231) cells. Catechol treatment showed concentration-dependent cytotoxicity and antiproliferative growth in both MCF-7 and MDA-MB-231 cells while sparing minimal effects on noncancerous (F-180 and HK2) cells. Catechol modulated differential DNA damage effects by activating ATM/ATR pathways and showed enhanced gamma-H2AX expression, as an indicator for DNA double-stranded breaks. MCF-7 cells showed G1 cell cycle arrest by regulating p21-mediated cyclin E/Cdk2 inhibition. Furthermore, activation of p53 triggered a caspase-mediated cell death mechanism by inhibiting regulatory proteins such as DNMT1, p-BRCA1, MCL-1, and PDCD6 with an increased Bax/Bcl-2 ratio. Overall, our results showed that catechol possesses favorable safety profile for noncancerous cells while specifically targeting multiple signaling cascades to inhibit proliferation in breast cancer cells.
- Journal
- Phytother Res
- Publish Year
- 2021
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Breast Cancers; Breast Cancer
- Paper Title Cn
- Paper Title En
- Natural compound catechol induces DNA damage, apoptosis, and G1 cell cycle arrest in breast cancer cells
- Bilingual Status
- semi_complete