ReferenceID 964
Protective effect of Hovenia dulcis Thunb. leaf extracts against ethanol-induced DNA damage in SH-SY5Y cells
J Ethnopharmacol
Ethnopharmacological relevance: Hovenia dulcis Thunb. has been used as a medicinal herb for the treatment of hepatic diseases and alcohol intoxication. Aim of the study: The genotoxic effect and the antigenotoxic potenti
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Record Fields
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- Reference Id
- 964
- Evidence Id
- 17554
- Core Evidence Id
- 17554
- Source Reference Id
- 1920
- Herb2 Reference Id
- HBREF002717
- Subject Paper Key
- HERB007001_36529249
- Pubmed Id
- 36529249
- Doi
- 10.1016/j.jep.2022.116042
- Paper Title
- Protective effect of Hovenia dulcis Thunb. leaf extracts against ethanol-induced DNA damage in SH-SY5Y cells
- Paper Abstract
- Ethnopharmacological relevance: Hovenia dulcis Thunb. has been used as a medicinal herb for the treatment of hepatic diseases and alcohol intoxication. Aim of the study: The genotoxic effect and the antigenotoxic potential of ethanolic extract of H. dulcis leaves and its methanolic fraction were evaluated against ethanol-induced damages in SH-SY5Y cells. Materials and methods: The phytochemical analysis and antioxidant activity of H. dulcis extracts were also assessed. In addition, a systems biology analysis was performed to investigate the molecular pathway of action of the H. dulcis leaves compounds. Results: The ethanolic extract and its methanolic fraction presented genotoxicity through comet assay at 0.5 and 0.25 mg/mL. On the other hand, both extracts showed protective action against ethanol at all concentrations. Additionally, an NBT assay was performed and demonstrated an ability of the extracts to reduce superoxide anion formation when SH-SY5Y cells were challenged with ethanol. HPLC analysis indicated the presence of quercitrin, isoquercitrin, and rutin. Further, system biology assays indicated a molecular action pathway, where the compounds from the leaves of H. dulcis, in addition to performing free radical scavenging activity, activate PP2A, and may inhibit the apoptosis pathway activated by ethanol-induced oxidative stress. Conclusions: This work is important to indicate potential antigenotoxic and antioxidant properties of H. dulcis leaves, and its use can be investigated against DNA damage induced by ethanol.
- Journal
- J Ethnopharmacol
- Publish Year
- 2022
- Experiment Subject
- sh-sy5y cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Hepatic Diseases; Alcohol Intoxication
- Paper Title Cn
- Paper Title En
- Protective effect of Hovenia dulcis Thunb. leaf extracts against ethanol-induced DNA damage in SH-SY5Y cells
- Bilingual Status
- semi_complete