ReferenceID 955
Extract from the Coriolus versicolor Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells
Int J Mol Sci
Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term us
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Record Fields
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- Reference Id
- 955
- Evidence Id
- 17545
- Core Evidence Id
- 17545
- Source Reference Id
- 1906
- Herb2 Reference Id
- HBREF002703
- Subject Paper Key
- HERB006860_33260615
- Pubmed Id
- 33260615
- Doi
- 10.3390/ijms21239063
- Paper Title
- Extract from the Coriolus versicolor Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells
- Paper Abstract
- Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term use of the currently available anti-inflammatory drugs used in cancer therapy causes numerous side effects, the aim of this study was to investigate the effect of an extract isolated from the Coriolus versicolor fungus (CV extract) on HUVEC endothelial cells and MCF-7 breast cancer cells in a pro-inflammatory microenvironment mimicked by lipopolysaccharide (LPS). The cells were simultaneously stimulated with the LPS and CV extract. After co-treatment, the cell viability, generation of reactive oxygen species (ROS), wound-healing assay, production of the pro-inflammatory and pro-angiogenic factors (interleukin (IL) 6, IL-8, and metalloproteinase (MMP) 9)), as well as expression of Toll-like receptor (TLR) 4 and phosphorylated IkappaB (p-IkappaB) were evaluated. The results showed that the CV extract inhibited IL-6, IL-8, and MMP-9 production by the LPS-stimulated cells. This effect was accompanied by a decrease in TLR4 and p-IkappaB expression. The CV extract also had anti-migratory properties and induced a cytotoxic effect on the cells that was enhanced in the presence of LPS. The observed cytotoxicity was associated with an increase in ROS generation. We conclude that the CV extract possesses cytotoxic activity against cancer cells and endothelial cells and has the ability to inhibit the expression of the pro-tumorigenic factors associated with inflammation.
- Journal
- Int J Mol Sci
- Publish Year
- 2020
- Experiment Subject
- huvec endothelial cells; lps-stimulated cells; mcf-7 breast cancer cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Chronic Inflammation; Inflammation; Cancer; Tumour; Breast Cancer
- Paper Title Cn
- Paper Title En
- Extract from the Coriolus versicolor Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells
- Bilingual Status
- semi_complete