ReferenceID 943
Protective Effect of Alpinia oxyphylla Fruit against tert-Butyl Hydroperoxide-Induced Toxicity in HepG2 Cells via Nrf2 Activation and Free Radical Scavenging and Its Active Molecules
Antioxidants (Basel)
Alpinia oxyphylla Miq. (Zingiberaceae) extract exerts protective activity against tert -butyl hydroperoxide-induced toxicity in HepG2 cells, and the antioxidant response element (ARE) luciferase activity increased 6-fold
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Record Fields
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- Reference Id
- 943
- Evidence Id
- 17533
- Core Evidence Id
- 17533
- Source Reference Id
- 1885
- Herb2 Reference Id
- HBREF002682
- Subject Paper Key
- HERB006569_35624896
- Pubmed Id
- 35624896
- Doi
- 10.3390/antiox11051032
- Paper Title
- Protective Effect of Alpinia oxyphylla Fruit against tert-Butyl Hydroperoxide-Induced Toxicity in HepG2 Cells via Nrf2 Activation and Free Radical Scavenging and Its Active Molecules
- Paper Abstract
- Alpinia oxyphylla Miq. (Zingiberaceae) extract exerts protective activity against tert -butyl hydroperoxide-induced toxicity in HepG2 cells, and the antioxidant response element (ARE) luciferase activity increased 6-fold at 30 μg/mL in HepG2 cells transiently transfected with ARE-luciferase. To identify active molecules, activity-guided isolation of the crude extract led to four sesquiterpenes ( 1 , 2 , 5 , 6 ) and two diarylheptanoids ( 3 and 4 ) from an n -hexane extract and six sesquiterpenes ( 7 - 12 ) from an ethyl acetate extract. Chemical structures were elucidated by one-dimensional, two-dimensional nuclear magnetic resonance (1D-, 2D-NMR), and mass (MS) spectral data. Among the isolated compounds, eudesma-3,11-dien-2-one ( 2 ) promoted the nuclear accumulation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and increased the promoter property of the ARE. Diarylheptanoids, yakuchinone A ( 3 ), and 5'-hydroxyl-yakuchinone A ( 4 ) showed radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Furthermore, optimization of extraction solvents (ratios of water and ethanol) was performed by comparison of contents of active compounds, ARE-inducing activity, radical scavenging activity, and HepG2 cell protective activity. As a result, 75% ethanol was the best solvent for the extraction of A. oxyphylla fruit. This study demonstrated that A. oxyphylla exerted antioxidant effects via the Nrf2/HO-1 (heme oxygenase-1) pathway and radical scavenging along with active markers eudesma-3,11-dien-2-one ( 2 ) and yakuchinone A ( 3 ).
- Journal
- Antioxidants (Basel)
- Publish Year
- 2022
- Experiment Subject
- hepg2 cell; hepg2 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Protective Effect of Alpinia oxyphylla Fruit against tert-Butyl Hydroperoxide-Induced Toxicity in HepG2 Cells via Nrf2 Activation and Free Radical Scavenging and Its Active Molecules
- Bilingual Status
- semi_complete