ReferenceID 87
BDNF mediates neuroprotection against oxygen-glucose deprivation by the cardiac glycoside oleandrin
J Neurosci
We have previously shown that the botanical drug candidate PBI-05204, a supercritical CO2 extract of Nerium oleander, provides neuroprotection in both in vitro and in vivo brain slice-based models for focal ischemia (Dun
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Record Fields
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- Reference Id
- 87
- Evidence Id
- 16677
- Core Evidence Id
- 16677
- Source Reference Id
- 147
- Herb2 Reference Id
- HBREF000320
- Subject Paper Key
- HBIN037934_24431454
- Pubmed Id
- 24431454
- Doi
- 10.1523/JNEUROSCI.2700-13.2014
- Paper Title
- BDNF mediates neuroprotection against oxygen-glucose deprivation by the cardiac glycoside oleandrin
- Paper Abstract
- We have previously shown that the botanical drug candidate PBI-05204, a supercritical CO2 extract of Nerium oleander, provides neuroprotection in both in vitro and in vivo brain slice-based models for focal ischemia (Dunn et al., 2011). Intriguingly, plasma levels of the neurotrophin BDNF were increased in patients treated with PBI-05204 in a phase I clinical trial (Bidyasar et al., 2009). We thus tested the hypothesis that neuroprotection provided by PBI-05204 to rat brain slices damaged by oxygen-glucose deprivation (OGD) is mediated by BDNF. We found, in fact, that exogenous BDNF protein itself is sufficient to protect brain slices against OGD, whereas downstream activation of TrkB receptors for BDNF is necessary for neuroprotection provided by PBI-05204, using three independent methods. Finally, we provide evidence that oleandrin, the principal cardiac glycoside component of PBI-05204, can quantitatively account for regulation of BDNF at both the protein and transcriptional levels. Together, these findings support further investigation of cardiac glycosides in providing neuroprotection in the context of ischemic stroke.
- Journal
- J Neurosci
- Publish Year
- 2014
- Experiment Subject
- rat
- Experiment Type
- Animal Experiment
- Phenotype Related
- Ischemic Stroke
- Paper Title Cn
- Paper Title En
- BDNF mediates neuroprotection against oxygen-glucose deprivation by the cardiac glycoside oleandrin
- Bilingual Status
- semi_complete