ReferenceID 818

C-geranylated flavonoids from Paulownia tomentosa Steud. fruit as potential anti-inflammatory agents

J Ethnopharmacol

Ethnopharmacological relevance: Paulownia tomentosa Steud., a traditional Chinese medicinal plant, was used for many centuries in Chinese herbal medicine as a component of remedies for many illnesses, including inflammat

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Reference Id
818
Evidence Id
17408
Core Evidence Id
17408
Source Reference Id
1633
Herb2 Reference Id
HBREF002430
Subject Paper Key
HERB003806_35760257
Pubmed Id
35760257
Doi
10.1016/j.jep.2022.115509
Paper Title
C-geranylated flavonoids from Paulownia tomentosa Steud. fruit as potential anti-inflammatory agents
Paper Abstract
Ethnopharmacological relevance: Paulownia tomentosa Steud., a traditional Chinese medicinal plant, was used for many centuries in Chinese herbal medicine as a component of remedies for many illnesses, including inflammatory diseases. It is a rich source of phenolic compounds, mainly geranylated flavonoids, which are currently studied for their promising biological activities. Aim of the study: The study aimed to isolate minor geranylated flavanones and flavones from P. tomentosa fruit and evaluate their cytotoxicity and possible anti-inflammatory effects in a cell-based model of inflammation. Materials and methods: Chromatographic separation of chloroform portion of the ethanolic extract of P. tomentosa fruit led to the isolation of twenty-seven flavonoids (1-27), twenty-six of them geranylated with different modifications and one non-geranylated flavanone, and two phenolic compounds. Compounds were identified using UV, IR, HRMS, NMR, and CD spectroscopy. Ten of these compounds (7-10, 12, 21, 22, 24, 25, and 27) were determined to be new flavonoid derivatives obtained from a natural source for the first time. Selected compounds were analyzed for cytotoxicity and anti-inflammatory potential to affect the activation of nuclear factor κB/activator protein 1 (NF-κB/AP-1) after lipopolysaccharide (LPS) stimulation. Results: All the test compounds (1-21 and 23-26) reduced the activation of NF-κB/AP-1 24 h after the addition of LPS. Eight compounds (5, 14-18, 21, and 26) were more active than prednisone, a widely used anti-inflammatory drug. However, this effect was not seen significantly on the level of TNF-α and IL-1β, which can be explained by the plurality of possible outcomes of activation of the NF-κB pathway in cells. Conclusions: Results of the presented study confirmed that constituents from traditional Chinese medicinal plant P. tomentosa Steud. have promising anti-inflammatory activities and can serve as a potential source of inspiration for new anti-inflammatory medications.
Journal
J Ethnopharmacol
Publish Year
2022
Experiment Subject
fruit
Experiment Type
Cell Experiment
Phenotype Related
Inflammatory Diseases; Inflammation
Paper Title Cn
Paper Title En
C-geranylated flavonoids from Paulownia tomentosa Steud. fruit as potential anti-inflammatory agents
Bilingual Status
semi_complete