ReferenceID 793
Indigo Pulverata Levis (Chung-Dae, Persicaria tinctoria) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro
Int J Mol Sci
Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The Indigo Pulverata Levis extract (CHD) is used in traditional Southeast Asian medicine; howeve
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Record Fields
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- Reference Id
- 793
- Evidence Id
- 17383
- Core Evidence Id
- 17383
- Source Reference Id
- 1574
- Herb2 Reference Id
- HBREF002371
- Subject Paper Key
- HERB003401_35008979
- Pubmed Id
- 35008979
- Doi
- 10.3390/ijms23010553
- Paper Title
- Indigo Pulverata Levis (Chung-Dae, Persicaria tinctoria) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro
- Paper Abstract
- Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The Indigo Pulverata Levis extract (CHD) is used in traditional Southeast Asian medicine; however, its beneficial effects on AD remain uninvestigated. Therefore, we investigated the therapeutic effects of CHD in 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice and tumor necrosis factor (TNF)-α- and interferon gamma (IFN)-γ-stimulated HaCaT cells. We evaluated immune cell infiltration, skin thickness, and the serum IgE and TNF-α levels in DNCB-induced AD mice. Moreover, we measured the expression levels of pro-inflammatory cytokines, mitogen-activated protein kinase (MAPK), and the nuclear factor-kappa B (NF-κB) in the mice dorsal skin. We also studied the effect of CHD on the translocation of NF-κB p65 and inflammatory chemokines in HaCaT cells. Our in vivo results revealed that CHD reduced the dermis and epidermis thicknesses and inhibited immune cell infiltration. Furthermore, it suppressed the proinflammatory cytokine expression and MAPK and NF-κB phosphorylations in the skin tissue and decreased serum IgE and TNF-α levels. In vitro results indicated that CHD downregulated inflammatory chemokines and blocked NF-κB p65 translocation. Thus, we deduced that CHD is a potential drug candidate for AD treatment.
- Journal
- Int J Mol Sci
- Publish Year
- 2022
- Experiment Subject
- mouse; hacat cells; tumor necrosis factor (tnf)-α- and interferon gamma (ifn)-γ-stimulated hacat cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Type 2 T; Chronic Inflammatory Skin Disease; Tumor; Atopic Dermatitis
- Paper Title Cn
- Paper Title En
- Indigo Pulverata Levis (Chung-Dae, Persicaria tinctoria) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro
- Bilingual Status
- semi_complete