ReferenceID 792
Protective effect of sterols extracted from Lotus plumule on ethanol-induced injury in GES-1 cells in vitro
Food Funct
In this study, sterols were isolated from Lotus plumule by Soxhlet extraction and saponification and were further characterized by GC-MS analysis. The results showed that the sterols extracted from Lotus plumule mainly c
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Record Fields
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- Reference Id
- 792
- Evidence Id
- 17382
- Core Evidence Id
- 17382
- Source Reference Id
- 1572
- Herb2 Reference Id
- HBREF002369
- Subject Paper Key
- HERB003378_34821900
- Pubmed Id
- 34821900
- Doi
- 10.1039/d1fo02684d
- Paper Title
- Protective effect of sterols extracted from Lotus plumule on ethanol-induced injury in GES-1 cells in vitro
- Paper Abstract
- In this study, sterols were isolated from Lotus plumule by Soxhlet extraction and saponification and were further characterized by GC-MS analysis. The results showed that the sterols extracted from Lotus plumule mainly contained beta-sitosterol, fucosterol, and campesterol. Models were established in vitro to investigate the protective effects of Lotus plumule sterols (LPSs) on ethanol-induced injury in human gastric epithelium (GES-1) cells. The results showed that appropriate concentrations of LPSs and beta-sitosterol could protect GES-1 cells from ethanol-induced injury by reducing ROS levels, reducing calcium ion release, increasing antioxidant enzyme activity and maintaining mitochondrial membrane potential. Western blot experiment results also showed that appropriate concentrations of LPSs and beta-sitosterol could up-regulate the expression of the anti-apoptotic protein Bcl-2 and down-regulate the pro-apoptotic proteins Bax and caspase-3 in GES-1 cells. Meanwhile, sterol pretreatment groups down-regulated the protein expression levels of p-P38 and p-JNK in ethanol-damaged GES-1 cells and up-regulated the expression level of p-ERK, suggesting that sterols protect GES-1 cells from ethanol-induced damage by regulating the MAPK signaling pathway. Taken together, Lotus plumule sterols could effectively prevent gastric cell damage in vitro and suggest the potential application of LPSs as bioactive ingredients for healthy foods.
- Journal
- Food Funct
- Publish Year
- 2021
- Experiment Subject
- human; ethanol-damaged ges-1 cells; ges-1 cells; human gastric epithelium (ges-1) cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Protective effect of sterols extracted from Lotus plumule on ethanol-induced injury in GES-1 cells in vitro
- Bilingual Status
- semi_complete