ReferenceID 766
Artemisia extracts differ from artemisinin effects on human hepatic CYP450s 2B6 and 3A4 in vitro
J Ethnopharmacol
Ethnopharmacological relevance: The Chinese medicinal herb, Artemisia annua L., has been used for >2,000 yr as traditional tea infusions to treat a variety of infectious diseases including malaria, and its use is spreadi
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Record Fields
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- Reference Id
- 766
- Evidence Id
- 17356
- Core Evidence Id
- 17356
- Source Reference Id
- 1511
- Herb2 Reference Id
- HBREF002308
- Subject Paper Key
- HERB002515_35934190
- Pubmed Id
- 35934190
- Doi
- 10.1016/j.jep.2022.115587
- Paper Title
- Artemisia extracts differ from artemisinin effects on human hepatic CYP450s 2B6 and 3A4 in vitro
- Paper Abstract
- Ethnopharmacological relevance: The Chinese medicinal herb, Artemisia annua L., has been used for >2,000 yr as traditional tea infusions to treat a variety of infectious diseases including malaria, and its use is spreading globally (along with A. afra Jacq. ex Willd.) mainly through grassroots efforts. Aim of the study: Artemisinin is more bioavailable delivered from the plant, Artemisia annua L. than the pure drug, but little is known about how delivery via a hot water infusion (tea) alters induction of hepatic CYP2B6 and CYP3A4 that metabolize artemisinin. Materials and methods: HepaRG cells were treated with 10 μM artemisinin or rifampicin (positive control), and teas (10 g/L) of A. annua SAM, and A. afra SEN and MAL with 1.6, 0.05 and 0 mg/g DW artemisinin in the leaves, respectively; qPCR and Western blots were used to measure CYP2B6 and CYP3A4 responses. Enzymatic activity of these P450s was measured using human liver microsomes and P450-Glo assays. Results: All teas inhibited activity of CYP2B6 and CYP3A4. Artemisinin and the high artemisinin-containing tea infusion (SAM) induced CYP2B6 and CYP3A4 transcription, but artemisinin-deficient teas, MAL and SEN, did not. Artemisinin increased CYP2B6 and CYP3A4 protein levels, but none of the three teas did, indicating a post-transcription inhibition by all three teas. Conclusions: This study showed that Artemisia teas inhibit activity and artemisinin autoinduction of CYP2B6 and CYP3A4 post transcription, a response likely the effect of other phytochemicals in these teas. Results are important for understanding Artemisia tea posology.
- Journal
- J Ethnopharmacol
- Publish Year
- 2022
- Experiment Subject
- human; heparg cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Malaria; Infectious Diseases
- Paper Title Cn
- Paper Title En
- Artemisia extracts differ from artemisinin effects on human hepatic CYP450s 2B6 and 3A4 in vitro
- Bilingual Status
- semi_complete