ReferenceID 763
Polygonum cuspidatum Extract Exerts Antihyperlipidemic Effects by Regulation of PI3K/AKT/FOXO3 Signaling Pathway
Oxid Med Cell Longev
Polygonum cuspidatum (PC) has been reported to exert a potent antihyperlipidemic effect. However, its mechanisms of action and active ingredients remain elusive and require further research. In this study, we first condu
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Record Fields
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- Reference Id
- 763
- Evidence Id
- 17353
- Core Evidence Id
- 17353
- Source Reference Id
- 1504
- Herb2 Reference Id
- HBREF002301
- Subject Paper Key
- HERB002407_34925692
- Pubmed Id
- 34925692
- Doi
- 10.1155/2021/3830671
- Paper Title
- Polygonum cuspidatum Extract Exerts Antihyperlipidemic Effects by Regulation of PI3K/AKT/FOXO3 Signaling Pathway
- Paper Abstract
- Polygonum cuspidatum (PC) has been reported to exert a potent antihyperlipidemic effect. However, its mechanisms of action and active ingredients remain elusive and require further research. In this study, we first conducted in vivo experiments to validate that Polygonum cuspidatum extract (PCE) could ameliorate the blood lipid level in hyperlipidemia model rats. Then, ultrahigh performance liquid chromatography coupled with Q-Exactive MS/MS (UPLC-QE-MS/MS) was applied to verify its 12 main active ingredients. The pharmacophore matching model was employed to predict the target point of the active ingredient, and 27 overlapping genes were identified via database and literature mining. String online database and Cytoscape software were utilized to construct a Protein-Protein Interaction (PPI) network, followed by function annotation analysis and pathway enrichment analysis. The results showed that the PI3K/AKT signaling pathway and its downstream FOXO3/ERalpha factors were significantly enriched. Furthermore, in vitro experiments were performed to determine the lipid content and oxidative stress (OS) indicators in OA-induced HepG2 cells, and immunofluorescence and western blotting analysis were carried out to analyze the effects of PCE on related proteins. Our experimental results show that the mechanism of antihyperlipidemic action of PCE is related to the activation of the PI3K/AKT signaling pathway and its downstream FOXO3/ERalpha factors, and polydatin and resveratrol are the main active ingredients in PCE that exert antihyperlipidemic effects.
- Journal
- Oxid Med Cell Longev
- Publish Year
- 2021
- Experiment Subject
- rat; hepg2 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Hyperlipidemia
- Paper Title Cn
- Paper Title En
- Polygonum cuspidatum Extract Exerts Antihyperlipidemic Effects by Regulation of PI3K/AKT/FOXO3 Signaling Pathway
- Bilingual Status
- semi_complete