ReferenceID 761
The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut ( Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats
Nutrients
The study investigated the protective effect of walnut oligopeptides (WOPs) against ethanol-induced gastric injury using Sprague-Dawley (SD) rats. Rats were randomly divided into seven groups based on body weight (10/gro
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Record Fields
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- Reference Id
- 761
- Evidence Id
- 17351
- Core Evidence Id
- 17351
- Source Reference Id
- 1502
- Herb2 Reference Id
- HBREF002299
- Subject Paper Key
- HERB002399_32325708
- Pubmed Id
- 32325708
- Doi
- 10.3390/nu12041138
- Paper Title
- The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut ( Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats
- Paper Abstract
- The study investigated the protective effect of walnut oligopeptides (WOPs) against ethanol-induced gastric injury using Sprague-Dawley (SD) rats. Rats were randomly divided into seven groups based on body weight (10/group), normal group, ethanol group, whey protein group (220 mg/kg body weight), omeprazole group (20 mg/kg body weight), and three WOPs groups (220, 440, 880 mg/kg body weight). After 30 days of treatment with WOPs, rats were given 5 ml/kg absolute ethanol by gavage to induce gastric mucosal injury. Gastric ulcer index (GUI) were determined and the following measured; gastric content pH, gastric mucin, endogenous pepsinogens (PG), prostaglandin E2 (PGE2), inflammatory cytokines, oxidative stress indicators, and the expression of apoptosis-related proteins were measured to evaluate the gastroprotective effect of WOPs. The results showed that the administration with WOPs markedly mitigated the hemorrhagic gastric lesions caused by ethanol in rats, and decreased the GUI, the gastric content pH, PG1, PG2, and NO levels, enhanced mucin and PGE2. Also, WOPs repressed gastric inflammation through the reduction of TNF-alpha, IL-6, IL-1beta and increase IL-10 levels, and revealed antioxidant properties with the enhancement of superoxide dismutase, glutathione, and catalase activity, while reduction of malondialdehyde. Moreover, WOPs treatment significantly down-regulated Bax, caspase-3 and nuclear factor-kappaB p65 (NF-kappaB p65) expression, while up-regulating the expression of Bcl-2 and inhibitor kappa Balpha (IkappaBalpha) protein. These results indicated that WOPs have protective effects against ethanol-induced gastric mucosal injury in rats through anti-inflammatory, anti-oxidation, and anti-apoptosis mechanisms.
- Journal
- Nutrients
- Publish Year
- 2020
- Experiment Subject
- rat
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Gastric Mucosal Injury; Gastric Ulcer; Ethanol-induced Gastric Mucosal Injury; Hemorrhagic Gastric Lesions; Gastric Injury
- Paper Title Cn
- Paper Title En
- The Gastroprotective Effect of Small Molecule Oligopeptides Isolated from Walnut ( Juglans regia L.) against Ethanol-Induced Gastric Mucosal Injury in Rats
- Bilingual Status
- semi_complete