ReferenceID 759

Preclinical evaluation of Zanthoxylum piperitum Benn., traditional muscle pain remedy, for joint inflammation

J Ethnopharmacol

Ethnopharmacological relevance: Zanthoxylum piperitum has been used as a traditional Asian medicine to treat hypertension, stroke, bruise and muscle pain. It has been known to induce detoxification; affect anti-bacterial

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Reference Id
759
Evidence Id
17349
Core Evidence Id
17349
Source Reference Id
1497
Herb2 Reference Id
HBREF002294
Subject Paper Key
HERB002372_34921962
Pubmed Id
34921962
Doi
10.1016/j.jep.2021.114921
Paper Title
Preclinical evaluation of Zanthoxylum piperitum Benn., traditional muscle pain remedy, for joint inflammation
Paper Abstract
Ethnopharmacological relevance: Zanthoxylum piperitum has been used as a traditional Asian medicine to treat hypertension, stroke, bruise and muscle pain. It has been known to induce detoxification; affect anti-bacterial, anti-oxidant, and tyrosinase activity; inhibit osteosarcoma proliferation; anti-osteoarthritis inflammation. In this study, we aim to identify the therapeutic effect of Z. piperitum 90% EtOH extract (ZPE-LR) on rheumatoid arthritis. Material and methods: We investigated the anti-rheumatoid arthritis and -immunomodulatory activities of the ZPE-LR in collagen-induced arthritic (CIA) mice, a rheumatoid arthritis animal model. In order to assess the analgesic effects of ZPE-LR in vivo, acetic acid injection, formaldehyde injection, hot plate model was used. The mechanism for anti-inflammatory activity of ZPE-LR was identified with LPS-stimulated Raw 264.7 cells. Results: Pharmacologically, oral administration of ZPE-LR into CIA mice resulted in a significant and dose-dependent decrease in clinical arthritis score and paw swelling compared to untreated negative control. Pathologic examination showed that ZPE-LR prevented morphological change in cartilage and destruction of phalanges in CIA mice. This protective effect was associated with reduced pain, inflammatory mediators such as NO, TNF-α, IL-1β, and IL-6, as well as COX-2 and iNOS expression. Furthermore, reduction of phosphor-ERK and BDNF indicates a novel rheumatoid arthritis-regulating mechanism by ZPE-LR treatment. Conclusions: These data suggest that the administration of ZPE-LR remarkably inhibited CIA progression and might be helpful in suppressing inflammation and pain in rheumatoid arthritis.
Journal
J Ethnopharmacol
Publish Year
2021
Experiment Subject
mouse; raw 264.7 cells
Experiment Type
Animal Experiment
Phenotype Related
Stroke; Arthritis; Collagen-induced Arthritic; Rheumatoid Arthritis; Muscle Pain; Anti-osteoarthritis Inflammation; Hypertension; Paw Swelling; Anti-rheumatoid Arthritis; Osteosarcoma; Bruise
Paper Title Cn
Paper Title En
Preclinical evaluation of Zanthoxylum piperitum Benn., traditional muscle pain remedy, for joint inflammation
Bilingual Status
semi_complete