ReferenceID 6561
Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
Front Pharmacol
Danlou tablet (DLT), a commercial Chinese patent medicine, has been widely used to treat cardiovascular diseases for many years. Atherosclerosis (AS) is the leading cause of cardiovascular disease. Increasing evidence in
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Record Fields
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- Reference Id
- 6561
- Evidence Id
- 23151
- Core Evidence Id
- 23151
- Source Reference Id
- 6422
- Herb2 Reference Id
- HBREF007219
- Subject Paper Key
- HBFO000766_34867337
- Pubmed Id
- 34867337
- Doi
- 10.3389/fphar.2021.730525
- Paper Title
- Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
- Paper Abstract
- Danlou tablet (DLT), a commercial Chinese patent medicine, has been widely used to treat cardiovascular diseases for many years. Atherosclerosis (AS) is the leading cause of cardiovascular disease. Increasing evidence indicates that autophagy plays a vital role in the development of AS. Here we investigated whether DLT could activate autophagy to improve AS and further clarified its underlying mechanisms. In an ApoE-/- mice model, the results of Oil red O, Masson's trichrome, and H&E staining techniques showed that DLT significantly inhibited lipid accumulation and fibrosis formation in atherosclerotic plaque tissue. DLT also inhibited serum triglyceride, cholesterol, and low-density lipoprotein levels and suppressed serum levels of inflammatory factors interleukin-6 and tumor necrosis factor-alpha in ApoE-/- mice. Moreover, DLT suppressed proliferation, migration, and invasion of human vascular adventitial fibroblasts (HVAFs) by inhibiting the PI3K/Akt/mTOR pathway. In addition, western blot analysis showed that Danlou tablet treatment decreased the expression of p62 and increased Beclin 1 and LC3 I -to-LC3 II ratios in HVAFs. The role of autophagy in treating atherosclerosis by DLT is confirmed by 3-methyladenine (autophagy inhibitor) and rapamycin (autophagy activator) in HVAFs. In summary, DLT activated PI3K/Akt/mTOR-mediated autophagy of vascular adventitial fibroblasts to protect cells from damage caused by atherosclerosis.
- Journal
- Front Pharmacol
- Publish Year
- 2021
- Experiment Subject
- mouse; human; hvafs
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Cardiovascular Disease; Atherosclerosis; Cardiovascular Diseases; Fibrosis; Atherosclerotic Plaque
- Paper Title Cn
- Paper Title En
- Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
- Bilingual Status
- semi_complete