ReferenceID 6560
Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent
Pharmacol Res
As a chronic inflammatory disease, atherosclerosis is characterized by accumulation of lipid-rich macrophages on the inner walls of arteries. Deposited macrophages promote atherosclerotic lesion progression; therefore th
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- Reference Id
- 6560
- Evidence Id
- 23150
- Core Evidence Id
- 23150
- Source Reference Id
- 6421
- Herb2 Reference Id
- HBREF007218
- Subject Paper Key
- HBFO000766_31181336
- Pubmed Id
- 31181336
- Doi
- 10.1016/j.phrs.2019.104306
- Paper Title
- Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent
- Paper Abstract
- As a chronic inflammatory disease, atherosclerosis is characterized by accumulation of lipid-rich macrophages on the inner walls of arteries. Deposited macrophages promote atherosclerotic lesion progression; therefore they are viewed as the main targets in order to alleviate atherosclerosis. Danlou tablet, a patented Chinese Medicine, has long been used to treat cardiovascular diseases. In the present study, we used Apolipoprotein E-deficient (ApoE-/-) mice model and in vitro cell line of RAW264.7 to explore the mechanisms of ethanol extracts of Danlou tablet (EEDT) in treating atherosclerosis. The potential targets that EEDT works to treat atherosclerosis were predicted by "Network pharmacology analysis", based on which we designed mRNA array of 93 genes. Then mRNA array and oil red O staining were performed in aortic extracted from the cohorts of Control (C57BL/6 mice, chow fed), Model (ApoE-/- C57BL/6 mice, 20 weeks of high-fat diet) and EEDT intervening (ApoE-/- mice, 20 weeks of high-fat diet with 12 weeks of EEDT treatment) group. Furthermore, mRNA array, inflammation cytokines and lipid content were examined in RAW264.7 cell line. It was showed that EEDT decreased the expressions of inflammation cytokines by down regulating NF-kappaB singling pathway and accelerated cholesterol effluent through activating PPARalpha/ABCA1 signaling pathway. On the other hand, activation of NF-kappaB pathway or suppression of PPARalpha/ABCA1 signaling pathway both abolished the therapeutic effect of EEDT. In conclusion, EEDT played a key role in anti-inflammation and preventing lipid deposition in macrophages of atherosclerosis via suppressing NF-kappaB signaling and triggering PPARalpha/ABCA1 signaling pathway.
- Journal
- Pharmacol Res
- Publish Year
- 2019
- Experiment Subject
- mouse; in vitro cell line; raw264.7 cell line
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Atherosclerosis; Cardiovascular Diseases; Chronic Inflammatory Disease; Atherosclerotic Lesion
- Paper Title Cn
- Paper Title En
- Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent
- Bilingual Status
- semi_complete