ReferenceID 6525
Zerumbone prevents pressure overload-induced left ventricular systolic dysfunction by inhibiting cardiac hypertrophy and fibrosis
Phytomedicine
BACKGROUND: Cardiac hypertrophy and fibrosis are hallmarks of cardiac remodeling and are involved functionally in the development of heart failure (HF). However, it is unknown whether Zerumbone (Zer) prevents left ventri
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- Reference Id
- 6525
- Evidence Id
- 23115
- Core Evidence Id
- 23115
- Source Reference Id
- 6344
- Herb2 Reference Id
- HBREF007141
- Subject Paper Key
- HBIN048883_34563985
- Pubmed Id
- 34563985
- Doi
- 10.1016/j.phymed.2021.153744
- Paper Title
- Zerumbone prevents pressure overload-induced left ventricular systolic dysfunction by inhibiting cardiac hypertrophy and fibrosis
- Paper Abstract
- BACKGROUND: Cardiac hypertrophy and fibrosis are hallmarks of cardiac remodeling and are involved functionally in the development of heart failure (HF). However, it is unknown whether Zerumbone (Zer) prevents left ventricular (LV) systolic dysfunction by inhibiting cardiac hypertrophy and fibrosis. PURPOSE: This study investigated the effect of Zer on cardiac hypertrophy and fibrosis in vitro and in vivo. STUDY DESIGN/METHODS: In primary cultured cardiac cells from neonatal rats, the effect of Zer on phenylephrine (PE)-induced hypertrophic responses and transforming growth factor beta (TGF-beta)-induced fibrotic responses was observed. To determine whether Zer prevents the development of pressure overload-induced HF in vivo, a transverse aortic constriction (TAC) mouse model was utilized. Cardiac function was evaluated by echocardiography. The changes of cardiomyocyte surface area were observed using immunofluorescence staining and histological analysis (HE and WGA staining). Collagen synthesis and fibrosis formation were measured by scintillation counter and picrosirius staining, respectively. The total mRNA levels of genes associated with hypertrophy (ANF and BNP) and fibrosis (Postn and alpha-SMA) were measured by qRT-PCR. The protein expressions (Akt and alpha-SMA) were assessed by western blotting. RESULTS: Zer significantly suppressed PE-induced increase in cell size, mRNA levels of ANF and BNP, and Akt phosphorylation in cardiomyocytes. The TGF-beta-induced increase in proline incorporation, mRNA levels of Postn and alpha-SMA, and protein expression of alpha-SMA were decreased by Zer in cultured cardiac fibroblasts. In the TAC male C57BL/6 mice, echocardiography results demonstrated that Zer improved cardiac function by increasing LV fractional shortening and reducing LV wall thickness compared with the vehicle group. ZER significantly reduced the level of phosphorylated Akt both in cultured cardiomyocytes treated with PE and in the hearts of TAC. Finally, Zer inhibited the pressure overload-induced cardiac hypertrophy and cardiac fibrosis. CONCLUSION: Zer ameliorates pressure overload-induced LV dysfunction, at least in part by suppressing both cardiac hypertrophy and fibrosis.
- Journal
- Phytomedicine
- Publish Year
- 2021
- Experiment Subject
- mouse; rat; cultured cardiac fibroblasts; cultured cardiomyocytes
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Lv Dysfunction; Heart Failure; Cardiac Hypertrophy; Transverse Aortic Constriction; Cardiac Fibrosis; Cardiac Remodeling; Fibrosis; Left Ventricular (lv) Systolic Dysfunction; Hypertrophy
- Paper Title Cn
- Paper Title En
- Zerumbone prevents pressure overload-induced left ventricular systolic dysfunction by inhibiting cardiac hypertrophy and fibrosis
- Bilingual Status
- semi_complete