ReferenceID 6483
Molecular mechanism of anti-adipogenic effect of vitexin in differentiating hMSCs
Phytother Res
In this study, we evaluated a detailed molecular mechanism of anti-adipogenic activity of vitexin, apigenin flavone glucoside, present in germinated fenugreek seeds, in differentiating human mesenchymal stem cells (hMSCs
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Record Fields
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- Reference Id
- 6483
- Evidence Id
- 23073
- Core Evidence Id
- 23073
- Source Reference Id
- 6270
- Herb2 Reference Id
- HBREF007067
- Subject Paper Key
- HBIN048102_34612537
- Pubmed Id
- 34612537
- Doi
- 10.1002/ptr.7300
- Paper Title
- Molecular mechanism of anti-adipogenic effect of vitexin in differentiating hMSCs
- Paper Abstract
- In this study, we evaluated a detailed molecular mechanism of anti-adipogenic activity of vitexin, apigenin flavone glucoside, present in germinated fenugreek seeds, in differentiating human mesenchymal stem cells (hMSCs). The lipid content of differentiated adipocytes was estimated by ORO staining. Effect on mitotic clonal expansion was checked by cell cycle analysis. Expression of early and terminal adipocyte differentiation markers, anti- and pro-adipogenic transcription factors and signalling intermediates regulating them was evaluated at RNA and protein level. We found vitexin to be non-cytotoxic up to 20 muM at which intracellular lipid accumulation was significantly decreased. Cell cycle analysis suggested that vitexin does not affect mitotic clonal expansion. Expression of early and late differentiation markers, such as CEBPalpha, CEBPbeta, PPARgamma, FABP4, perilipin, adiponectin and Glut4 was significantly reduced in the presence of vitexin. Expression of KLF4 and KLF15, positive regulators of PPARgamma, was decreased, whereas that of negative regulators, namely KLF2, GATA2, miR20a, miR27a, miR27b, miR128, miR130a, miR130b, miR182 and miR548 increased with vitexin treatment. This effect was mediated by the activation of the AMP-activated protein kinase (AMPK) pathway via the activation of LepR and additionally by inhibiting ROS. Thus, our results showed that vitexin regulates the expression of PPARgamma and inhibits adipogenesis of hMSCs at an early stage of differentiation.
- Journal
- Phytother Res
- Publish Year
- 2021
- Experiment Subject
- human; differentiating human mesenchymal stem cells; fenugreek
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Molecular mechanism of anti-adipogenic effect of vitexin in differentiating hMSCs
- Bilingual Status
- semi_complete