ReferenceID 640
Effect of Paeoniae Radix Rubra (Paeonia lactiflora Pall.) extract on mucin secretion, gene expression in human airway epithelial cells
J Ethnopharmacol
Ethnopharmacological relevance: Paeoniae Radix Rubra (PRR), the root of Paeonia lactiflora Pall., is a traditional Chinese medicine which has the effects of regulating various inflammatory diseases, treating blood stasis
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Record Fields
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- Reference Id
- 640
- Evidence Id
- 17230
- Core Evidence Id
- 17230
- Source Reference Id
- 1265
- Herb2 Reference Id
- HBREF002062
- Subject Paper Key
- HERB000800_36436716
- Pubmed Id
- 36436716
- Doi
- 10.1016/j.jep.2022.115959
- Paper Title
- Effect of Paeoniae Radix Rubra (Paeonia lactiflora Pall.) extract on mucin secretion, gene expression in human airway epithelial cells
- Paper Abstract
- Ethnopharmacological relevance: Paeoniae Radix Rubra (PRR), the root of Paeonia lactiflora Pall., is a traditional Chinese medicine which has the effects of regulating various inflammatory diseases, treating blood stasis, and enhancing blood circulation. Aim of the study: This study examined whether Paeoniae Radix rubra extract (PRRE) and Paeoniflorin (PF) affect mucin production, gene expression including MUC5AC, and protein expression related to the ERK pathway induced by TNF-α from human airway epithelial cells. Materials and methods: NCI-H292 cells induced by TNF-α were treated with each agent. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction, staining, and enzyme-linked immunosorbent assay. Western blot was used to investigate the cell signaling pathways. Results: PRRE and PF inhibited the production of MUC5AC mucin protein and gene expression in TNF-α-induced H292 cells. In Western blot, PRRE was involved in protein expression related to the ERK pathway. Conclusions: Overall, PRRE effectively inhibited the MUC5AC, and inflammatory cytokines expression caused by TNF-α, which was closely involved in the ERK pathway. PRRE may have the potential for treating mucus producing respiratory inflammation.
- Journal
- J Ethnopharmacol
- Publish Year
- 2022
- Experiment Subject
- human; h292 cells; nci-h292 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Inflammatory Diseases; Blood Stasis; Respiratory Inflammation
- Paper Title Cn
- Paper Title En
- Effect of Paeoniae Radix Rubra (Paeonia lactiflora Pall.) extract on mucin secretion, gene expression in human airway epithelial cells
- Bilingual Status
- semi_complete