ReferenceID 6356
Mechanisms involved in the anti-tumor effects of Toosendanin in glioma cells
Cancer Cell Int
BACKGROUND: Toosendanin (TSN) is a triterpenoid compound mainly used as an ascaris repellant. Recent studies have shown that it possesses antitumor effects in many types of tumor cells. However, the effects of TSN on gli
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 6356
- Evidence Id
- 22946
- Core Evidence Id
- 22946
- Source Reference Id
- 5987
- Herb2 Reference Id
- HBREF006784
- Subject Paper Key
- HBIN046550_34530814
- Pubmed Id
- 34530814
- Doi
- 10.1186/s12935-021-02186-2
- Paper Title
- Mechanisms involved in the anti-tumor effects of Toosendanin in glioma cells
- Paper Abstract
- BACKGROUND: Toosendanin (TSN) is a triterpenoid compound mainly used as an ascaris repellant. Recent studies have shown that it possesses antitumor effects in many types of tumor cells. However, the effects of TSN on glioma cells have rarely been reported. METHODS: Different assays were performed to investigate the effects of TSN on the different glioma cell lines including U87MG and LN18. The assays included colony formation, wound healing, and transwell assays. Furthermore, Hoechst 33342 staining, flow cytometry, and western blotting analysis were performed to investigate the apoptotic activities of TSN. Finally, the results were confirmed using a xenograft tumor model that comprised of nude mice. RESULTS: In vitro, the CCK-8 and colony formation assays showed that TSN effectively inhibited glioma cell proliferation. Moreover, the inhibitory effects on glioma cell migration and invasion were demonstrated through the wound healing and transwell assays, respectively. Hoechst 33342 staining, flow cytometry, and western blotting assays demonstrated the significant effect of TSN in the apoptosis induction of glioma cells. Furthermore, the anti-glioma effect of TSN was exerted through the inhibition of the PI3K/Akt/mTOR signaling pathways as demonstrated by western blotting analysis. In addition, the effects of TSN on glioma cell viability, apoptosis, cell cycle arrest, migration, and invasion were reversed by 740Y-P, a PI3K activator. Finally, the mouse xenograft model confirmed the suppressive effect of TSN on tumor growth in vivo. CONCLUSION: Our results suggest that TSN is a promising chemotherapeutic drug for patients with glioma.
- Journal
- Cancer Cell Int
- Publish Year
- 2021
- Experiment Subject
- mouse; patient; glioma cell lines
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Tumor; Glioma
- Paper Title Cn
- Paper Title En
- Mechanisms involved in the anti-tumor effects of Toosendanin in glioma cells
- Bilingual Status
- semi_complete