ReferenceID 625
Purified Tea ( Camellia sinensis (L.) Kuntze) Flower Saponins Induce the p53-Dependent Intrinsic Apoptosis of Cisplatin-Resistant Ovarian Cancer Cells
Int J Mol Sci
Ovarian cancer is currently ranked at fifth in cancer deaths among women. Patients who have undergone cisplatin-based chemotherapy can experience adverse effects or become resistant to treatment, which is a major impedim
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- Reference Id
- 625
- Evidence Id
- 17215
- Core Evidence Id
- 17215
- Source Reference Id
- 1241
- Herb2 Reference Id
- HBREF002038
- Subject Paper Key
- HERB000619_32560563
- Pubmed Id
- 32560563
- Doi
- 10.3390/ijms21124324
- Paper Title
- Purified Tea ( Camellia sinensis (L.) Kuntze) Flower Saponins Induce the p53-Dependent Intrinsic Apoptosis of Cisplatin-Resistant Ovarian Cancer Cells
- Paper Abstract
- Ovarian cancer is currently ranked at fifth in cancer deaths among women. Patients who have undergone cisplatin-based chemotherapy can experience adverse effects or become resistant to treatment, which is a major impediment for ovarian cancer treatment. Natural products from plants have drawn great attention in the fight against cancer recently. In this trial, purified tea (Camellia sinensis (L.) Kuntze) flower saponins (PTFSs), whose main components are Chakasaponin I and Chakasaponin IV, inhibited the growth and proliferation of ovarian cancer cell lines A2780/CP70 and OVCAR-3. Flow cytometry, caspase activity and Western blotting analysis suggested that such inhibitory effects of PTFSs on ovarian cancer cells were attributed to the induction of cell apoptosis through the intrinsic pathway rather than extrinsic pathway. The p53 protein was then confirmed to play an important role in PTFS-induced intrinsic apoptosis, and the levels of its downstream proteins such as caspase families, Bcl-2 families, Apaf-1 and PARP were regulated by PTFS treatment. In addition, the upregulation of p53 expression by PTFSs were at least partly induced by DNA damage through the ATM/Chk2 pathway. The results help us to understand the mechanisms underlying the effects of PTFSs on preventing and treating platinum-resistant ovarian cancer.
- Journal
- Int J Mol Sci
- Publish Year
- 2020
- Experiment Subject
- patient; ovarian cancer cell lines; women
- Experiment Type
- Cell Experiment
- Phenotype Related
- Ptfss; Ovarian Cancer; Platinum-resistant Ovarian Cancer; Cancer
- Paper Title Cn
- Paper Title En
- Purified Tea ( Camellia sinensis (L.) Kuntze) Flower Saponins Induce the p53-Dependent Intrinsic Apoptosis of Cisplatin-Resistant Ovarian Cancer Cells
- Bilingual Status
- semi_complete