ReferenceID 6210
Chondro-Protective Effects of Shikimic Acid on Osteoarthritis via Restoring Impaired Autophagy and Suppressing the MAPK/NF-κB Signaling Pathway
Front Pharmacol
Osteoarthritis (OA) is a major cause of cartilage pain and limited mobility in middle-aged and elderly individuals. The degeneration of cartilage induced by inflammation and cartilage anabolic and catabolic disorder play
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Record Fields
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- Reference Id
- 6210
- Evidence Id
- 22800
- Core Evidence Id
- 22800
- Source Reference Id
- 5697
- Herb2 Reference Id
- HBREF006494
- Subject Paper Key
- HBIN043883_34385915
- Pubmed Id
- 34385915
- Doi
- 10.3389/fphar.2021.634822
- Paper Title
- Chondro-Protective Effects of Shikimic Acid on Osteoarthritis via Restoring Impaired Autophagy and Suppressing the MAPK/NF-κB Signaling Pathway
- Paper Abstract
- Osteoarthritis (OA) is a major cause of cartilage pain and limited mobility in middle-aged and elderly individuals. The degeneration of cartilage induced by inflammation and cartilage anabolic and catabolic disorder plays a key role in OA. Shikimic acid (SA), a natural ingredient extracted from Illicium verum, has been shown to exert notable anti-inflammatory effects in previous studies, suggesting its potential effects in the treatment of OA. In this study, we revealed that the pretreatment of SW1353 human chondrocytes with SA before interleukin 1beta (IL-1beta) stimulation effectively decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (Cox)-2, matrix metalloproteinases (MMPs; MMP3 and MMP13), a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-5, type X collagen, and p62; increased the expression of type II collagen, ATG7, Beclin-1, and LC3; and increased the autophagic flux. Mechanistically, we found that SA suppressed the IL-1beta-induced activation of the mitogen-activated protein kinase (MAPK) and nuclear factor-kappaB (NF-kappaB) pathways. Furthermore, the results of safranin O staining and toluidine blue staining of primary rat cartilage chondrocytes and a trauma-induced rat model of OA showed that SA alleviated progression of OA in vivo. Collectively, our research enhances understanding of the mechanism of protective effect of SA against the progression of OA, which involves amelioration of cartilage degeneration, thereby providing new evidence for the use of SA as a therapy to prevent the development of OA.
- Journal
- Front Pharmacol
- Publish Year
- 2021
- Experiment Subject
- rat; human; sw1353 human chondrocytes
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cartilage Anabolic And Catabolic Disorder; Degeneration Of Cartilage; Cartilage Pain; Cartilage Degeneration; Osteoarthritis
- Paper Title Cn
- Paper Title En
- Chondro-Protective Effects of Shikimic Acid on Osteoarthritis via Restoring Impaired Autophagy and Suppressing the MAPK/NF-κB Signaling Pathway
- Bilingual Status
- semi_complete