ReferenceID 6183
Thymol ameliorates ethanol-induced hepatotoxicity via regulating metabolism and autophagy
Chem Biol Interact
Alcoholic liver disease represents a serious threat to human health. In terms of safety and acceptability, thymol is widely used in or on foodstuffs to generate odour and taste. The present study aimed to investigate the
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 6183
- Evidence Id
- 22773
- Core Evidence Id
- 22773
- Source Reference Id
- 5977
- Herb2 Reference Id
- HBREF006774
- Subject Paper Key
- HBIN046387_36535314
- Pubmed Id
- 36535314
- Doi
- 10.1016/j.cbi.2022.110308
- Paper Title
- Thymol ameliorates ethanol-induced hepatotoxicity via regulating metabolism and autophagy
- Paper Abstract
- Alcoholic liver disease represents a serious threat to human health. In terms of safety and acceptability, thymol is widely used in or on foodstuffs to generate odour and taste. The present study aimed to investigate the therapeutic effect and mechanism of thymol against ethanol-induced injury in liver cells. Here we found that thymol is an effective agent for reducing ethanol-induced reactive oxygen species production in mouse liver cells. Thymol improves ethanol-induced lipid accumulation, and this corresponded to altered DGAT2 mRNA expression levels. Metabolomics data analysis showed that thymol alleviated ethanol-induced changes in the levels of thirty-four metabolites including nicotinic acid and l-arginine. By utilizing pathway enrichment analysis, altered metabolites in cells treated with ethanol and ethanol plus thymol were enriched in fourteen pathways including metabolic pathways and arginine and proline metabolism. We further confirmed the alleviation of overdose nitric oxide production in cells treated with ethanol plus thymol compared with that in ethanol-treated cells. It was interesting that up-regulated LC3-II/LC3-I ratio together with higher SQSTM1 protein abundance in ethanol-treated cells were attenuated by treatment with ethanol plus thymol. Thymol ameliorated ethanol-induced reduction of HSPA8 protein abundance. In addition, chloroquine-treated cells exhibited lower HSPA8 protein abundance compared with cells simulated with ethanol plus thymol. These data reveal that improving effect of thymol on ethanol-induced metabolic alteration is related to autophagic flux restoration. Our findings indicate that thymol is an attractive option for treating ethanol-induced liver damage.
- Journal
- Chem Biol Interact
- Publish Year
- 2022
- Experiment Subject
- mouse; human; chloroquine-treated cells; ethanol-treated cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Ethanol-induced Liver Damage; Alcoholic Liver Disease
- Paper Title Cn
- Paper Title En
- Thymol ameliorates ethanol-induced hepatotoxicity via regulating metabolism and autophagy
- Bilingual Status
- semi_complete