ReferenceID 6180
Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
Front Pharmacol
With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become the third major cause of iatrogenic renal insufficiency. CIN is defined as an absolute increase in seru
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Record Fields
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- Reference Id
- 6180
- Evidence Id
- 22770
- Core Evidence Id
- 22770
- Source Reference Id
- 5625
- Herb2 Reference Id
- HBREF006422
- Subject Paper Key
- HBIN042901_35035354
- Pubmed Id
- 35035354
- Doi
- 10.3389/fphar.2021.761908
- Paper Title
- Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
- Paper Abstract
- With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become the third major cause of iatrogenic renal insufficiency. CIN is defined as an absolute increase in serum creatinine levels of at least 0.50 mg/dl or an increase >25% of serum creatinine from baseline after exposure to contrast. In this study, the protective effects of salvianolic acid B (Sal B) were detected in human renal tubular epithelial cells (HK-2) exposed to iopromide. The results showed that different concentrations of Sal B counteract the loss of cell viability induced by iopromide, and reduce cell apoptosis, the reactive oxygen species (ROS) levels, and the levels of endoplasmic reticulum stress (ERS)-related and apoptosis-related proteins such as p-IRE-1alpha, p-eIF-2alpha/eIF-2alpha, p-JNK, CHOP, Bax/Bcl-2, and cleaved caspase-3. In addition, Sal B at a concentration of 100 mumol/L inhibited ERS and reduced cell damage to a similar extent as the ERS inhibitor 4-PBA. Importantly, treatment with Sal B could abolish the injury induced by ERS agonist tunicamycin, increasing cell viability and the mitochondrial membrane potential, as well as significantly reducing ROS levels and the expression of Bax/Bcl-2, cleaved-caspase-3, GRP78, p-eIF2alpha, p-JNK, and CHOP. These results suggested that the protective effect of Sal B against HK-2 cell injury induced by iopromide may be related to the inhibition of ERS.
- Journal
- Front Pharmacol
- Publish Year
- 2021
- Experiment Subject
- human
- Experiment Type
- Cell Experiment
- Phenotype Related
- Contrast-induced Nephropathy; Iatrogenic Renal Insufficiency
- Paper Title Cn
- Paper Title En
- Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
- Bilingual Status
- semi_complete