ReferenceID 6167
Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of Saikosaponin A
J Cell Mol Med
Depression is a commonly occurring neuropsychiatric disease with an increasing incidence rate. Saikosaponin A (SA), a major bioactive component extracted from Radix Bupleuri, possesses anti-malignant cell proliferation,
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- Reference Id
- 6167
- Evidence Id
- 22757
- Core Evidence Id
- 22757
- Source Reference Id
- 5600
- Herb2 Reference Id
- HBREF006397
- Subject Paper Key
- HBIN042787_31762213
- Pubmed Id
- 31762213
- Doi
- 10.1111/jcmm.14695
- Paper Title
- Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of Saikosaponin A
- Paper Abstract
- Depression is a commonly occurring neuropsychiatric disease with an increasing incidence rate. Saikosaponin A (SA), a major bioactive component extracted from Radix Bupleuri, possesses anti-malignant cell proliferation, anti-inflammation, anti-oxidation and liver protective effects. However, few studies have investigated SA's antidepressant effects and pharmacological mechanisms of action. Our study aimed to explore the anti-depression effect of SA and screen the target proteins regulated by SA in a rat model of chronic unpredictable mild stress (CUMS)-induced depression. Results showed that 8-week CUMS combined with separation could successfully produce depressive-like behaviours and cause a decrease of dopamine (DA) in rat hippocampus, and 4-week administration of SA could relieve CUMS rats' depressive symptoms and up-regulated DA content. There were 15 kinds of significant differentially expressed proteins that were detected not only between the control and CUMS groups, but also between the CUMS and SA treatment groups. Proline-rich transmembrane protein 2 (PRRT2) was down-regulated by CUMS while up-regulated by SA. These findings reveal that SA may exert antidepressant effects by up-regulating the expression level of PRRT2 and increasing DA content in hippocampus. The identification of these 15 differentially expressed proteins, including PRRT2, provides further insight into the treatment mechanism of SA for depression.
- Journal
- J Cell Mol Med
- Publish Year
- 2020
- Experiment Subject
- rat
- Experiment Type
- Animal Experiment
- Phenotype Related
- Chronic Unpredictable Mild Stress; Neuropsychiatric Disease
- Paper Title Cn
- Paper Title En
- Proteomics-based screening of the target proteins associated with antidepressant-like effect and mechanism of Saikosaponin A
- Bilingual Status
- semi_complete