ReferenceID 6164
Rutaecarpine alleviates acute pancreatitis in mice and AR42J cells by suppressing the MAPK and NF-κB signaling pathways via calcitonin gene-related peptide
Phytother Res
Acute pancreatitis (AP) is an acute inflammatory condition of the pancreas. Previous studies have shown that rutaecarpine (RUT), an important alkaloid component of Evodia rutaecarpa, exhibits certain protective effects a
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Record Fields
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- Reference Id
- 6164
- Evidence Id
- 22754
- Core Evidence Id
- 22754
- Source Reference Id
- 5591
- Herb2 Reference Id
- HBREF006388
- Subject Paper Key
- HBIN042657_34661951
- Pubmed Id
- 34661951
- Doi
- 10.1002/ptr.7301
- Paper Title
- Rutaecarpine alleviates acute pancreatitis in mice and AR42J cells by suppressing the MAPK and NF-κB signaling pathways via calcitonin gene-related peptide
- Paper Abstract
- Acute pancreatitis (AP) is an acute inflammatory condition of the pancreas. Previous studies have shown that rutaecarpine (RUT), an important alkaloid component of Evodia rutaecarpa, exhibits certain protective effects against AP in rats by upregulating calcitonin gene-related peptide (CGRP). However, the molecular mechanism of RUT in AP remains unknown. This study aimed to investigate the effects of RUT on cerulein-induced AP in vivo and in vitro, and to explore the underlying molecular mechanisms. In cerulein/LPS-treated wild-type mice, but not CGRP gene knock-out mice, RUT significantly ameliorated pancreatic inflammation by alleviating histopathological changes, reducing IL-6 and TNF-alpha levels, and increasing in IL-10 levels. Moreover, RUT improved AP by suppressing the MAPK and NF-kappaB signaling pathways. These effects were mostly mediated through CGRP. Cell-based studies revealed that RUT significantly improved cell viability while suppressing the apoptosis of AR42J cells with cerulein-induced AP, downregulating IL-6 and TNF-alpha, stimulating IL-10 release, and inhibiting MAPK, NF-kappaB, and STAT3 signaling activation, all in a CGRP-dependent manner. RUT ameliorated cerulein/LPS-induced AP inflammatory responses in mice and AR42J cells in a CGRP-dependent manner and thus may represent a potential therapeutic option for AP patients. Our study provides valuable insights for AP drug development.
- Journal
- Phytother Res
- Publish Year
- 2021
- Experiment Subject
- mouse; rat; patient; ar42j cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Pancreatic Inflammation; Acute Inflammatory Condition Of The Pancreas; Acute Pancreatitis
- Paper Title Cn
- Paper Title En
- Rutaecarpine alleviates acute pancreatitis in mice and AR42J cells by suppressing the MAPK and NF-κB signaling pathways via calcitonin gene-related peptide
- Bilingual Status
- semi_complete