ReferenceID 616
Angelica dahurica promoted angiogenesis and accelerated wound healing in db/db mice via the HIF-1α/PDGF-β signaling pathway
Free Radic Biol Med
Impaired angiogenesis is crucial for impeding the wound healing process in diabetic foot ulcers (DFUs). In this study, we found that Angelica dahurica (A. dahurica) stimulated angiogenesis and benefited wound healing in
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Record Fields
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- Reference Id
- 616
- Evidence Id
- 17206
- Core Evidence Id
- 17206
- Source Reference Id
- 1221
- Herb2 Reference Id
- HBREF002018
- Subject Paper Key
- HERB000305_32853721
- Pubmed Id
- 32853721
- Doi
- 10.1016/j.freeradbiomed.2020.08.015
- Paper Title
- Angelica dahurica promoted angiogenesis and accelerated wound healing in db/db mice via the HIF-1α/PDGF-β signaling pathway
- Paper Abstract
- Impaired angiogenesis is crucial for impeding the wound healing process in diabetic foot ulcers (DFUs). In this study, we found that Angelica dahurica (A. dahurica) stimulated angiogenesis and benefited wound healing in genetic mouse models of diabetes. In HUVECs, A. dahurica promoted cell proliferation and tube formation, which was accompanied by increased nuclear translocation of HIF-1alpha under hypoxic conditions and led to elevated PDGF-beta protein expression. A. dahurica activated the PI3K/AKT signaling pathway in human umbilical vein endothelial cells (HUVECs), which was abrogated by the PI3K inhibitor LY294002. Furthermore, the cellular expression of PDGF-beta decreased significantly after treatment with a HIF-1alpha-siRNA, and PDGF-beta expression was increased in HIF-1alpha-overexpressing cells. In a full-thickness cutaneous wound healing db/db mouse model, A. dahurica accelerated wound closure, which was reflected by a significantly reduced wound area and an increase in neovascularization, as well as by elevated PDGF-beta expression and increased capillary formation. In addition, A. dahurica activated the PI3K/AKT signaling pathway and enhanced HIF-1alpha synthesis in wounds. In summary, A. dahurica promoted angiogenesis of HUVECs in vitro by promoting signaling via the HIF-1alpha/PDGF-beta pathway, efficiently enhancing vascularization in regenerated tissue and facilitating wound healing in vivo. The results revealed that A. dahurica has potential as a therapy for vessel injury-related wounds.
- Journal
- Free Radic Biol Med
- Publish Year
- 2020
- Experiment Subject
- mouse; human; hif-1alpha-overexpressing cells; human umbilical vein endothelial cells; huvecs
- Experiment Type
- Animal Experiment
- Phenotype Related
- Dfus; Diabetes; Diabetic Foot Ulcers; Vessel Injury-related Wounds
- Paper Title Cn
- Paper Title En
- Angelica dahurica promoted angiogenesis and accelerated wound healing in db/db mice via the HIF-1α/PDGF-β signaling pathway
- Bilingual Status
- semi_complete