ReferenceID 6087
Puerarin sensitized K562/ADR cells by inhibiting NF-κB pathway and inducing autophagy
Phytother Res
Puerarin is an isoflavone isolated from Pueraria lobata (Willd.) Ohwi. In the present study, reversal effect and underlying mechanisms of puerarin on multidrug resistance (MDR) were investigated in K562/ADR cells. K562/A
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 6087
- Evidence Id
- 22677
- Core Evidence Id
- 22677
- Source Reference Id
- 5436
- Herb2 Reference Id
- HBREF006233
- Subject Paper Key
- HBIN041253_33141989
- Pubmed Id
- 33141989
- Doi
- 10.1002/ptr.6932
- Paper Title
- Puerarin sensitized K562/ADR cells by inhibiting NF-κB pathway and inducing autophagy
- Paper Abstract
- Puerarin is an isoflavone isolated from Pueraria lobata (Willd.) Ohwi. In the present study, reversal effect and underlying mechanisms of puerarin on multidrug resistance (MDR) were investigated in K562/ADR cells. K562/ADR cells exhibited adriamycin (ADR) resistance and higher levels of MDR1 expression compared with K562 cells. Puerarin enhanced the chemosensitivity of K562/ADR cells and increased the ADR accumulation in K562/ADR cells. The expression levels of MDR1 were down-regulated by puerarin in K562/ADR cells. Luciferase reporter assay further demonstrated the inhibitory effect of puerarin on TNF-alpha-induced NF-kappaB activation. The phosphorylation of IkappaB-alpha was significantly suppressed by puerarin. In silico docking analyses suggested that puerarin well matched with the active sites of IkappaB-alpha. Moreover, a large number of autophagosomes were found in the cytoplasm of K562/ADR cells after puerarin treatment. The significant increase in LC3-II and beclin-1 was also observed, indicating autophagy induction by puerarin in K562/ADR cells. Puerarin induced cell cycle arrest and apoptosis in K562/ADR cells. Finally, puerarin inhibited phosphorylation of Akt and JNK. In conclusion, puerarin-sensitized K562/ADR cells by downregulating MDR1 expression via inhibition of NF-kappaB pathway and autophagy induction via Akt inhibition.
- Journal
- Phytother Res
- Publish Year
- 2021
- Experiment Subject
- k562 cells; k562/adr cells; puerarin-sensitized k562/adr cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Puerarin sensitized K562/ADR cells by inhibiting NF-κB pathway and inducing autophagy
- Bilingual Status
- semi_complete