ReferenceID 6086
Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome
Life Sci
AIMS: Retinal ischemia/reperfusion (I/R) injury is common in the development of ophthalmic diseases and potentially causes blindness. In present study, the aim is to investigate the possible protective effects of puerari
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Record Fields
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- Reference Id
- 6086
- Evidence Id
- 22676
- Core Evidence Id
- 22676
- Source Reference Id
- 5432
- Herb2 Reference Id
- HBREF006229
- Subject Paper Key
- HBIN041253_32526286
- Pubmed Id
- 32526286
- Doi
- 10.1016/j.lfs.2020.117935
- Paper Title
- Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome
- Paper Abstract
- AIMS: Retinal ischemia/reperfusion (I/R) injury is common in the development of ophthalmic diseases and potentially causes blindness. In present study, the aim is to investigate the possible protective effects of puerarin on retinal I/R. MAIN METHODS: Retinal I/R injury was conducted on the left eyes of male Sprague Dawley rats, which were subsequently received treatment with puerarin. After administration, retinal I/R-induced apoptosis, oxidative stress and inflammatory responses were detected. Meanwhile, we purified retinal ganglion cells (RGCs) from 7-day-old rats. After subjected RGCs to oxygen and glucose deprivation/reoxygenation (OGD/R), apoptosis and TLR4/NLRP3 inflammasome activation in RGCs were detected. KEY FINDINGS: Puerarin prominently suppressed apoptosis, alleviated oxidative stress and suppressed TLR4/NLRP3 inflammasome activation in rats with retinal I/R injury. Consistent with our in vivo study, we found puerarin ameliorated retinal I/R injury through suppressing apoptosis and TLR4/NLRP3 inflammasome activation in RGCs. SIGNIFICANCE: Our findings reveal that puerarin plays a protective role against retinal I/R injury by alleviating RGC damage, and is beneficial for the treatment of I/R injury-caused ophthalmic diseases.
- Journal
- Life Sci
- Publish Year
- 2020
- Experiment Subject
- rat; sprague-dawley rat
- Experiment Type
- Animal Experiment
- Phenotype Related
- Blindness; Retinal Ischemia/reperfusion; Ophthalmic Diseases; Retinal I/r Injury; I/r Injury
- Paper Title Cn
- Paper Title En
- Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome
- Bilingual Status
- semi_complete