ReferenceID 608

Sanshool improves UVB-induced skin photodamage by targeting JAK2/STAT3-dependent autophagy

Cell Death Dis

Ultraviolet radiation is markedly increased because of pollution and the depletion of the stratospheric ozone layer. Excessive exposure to sunlight can negatively affect the skin, resulting in sunburn, photo-aging, or sk

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Reference Id
608
Evidence Id
17198
Core Evidence Id
17198
Source Reference Id
1199
Herb2 Reference Id
HBREF001996
Subject Paper Key
HBIN043050_30622245
Pubmed Id
30622245
Doi
10.1038/s41419-018-1261-y
Paper Title
Sanshool improves UVB-induced skin photodamage by targeting JAK2/STAT3-dependent autophagy
Paper Abstract
Ultraviolet radiation is markedly increased because of pollution and the depletion of the stratospheric ozone layer. Excessive exposure to sunlight can negatively affect the skin, resulting in sunburn, photo-aging, or skin cancer. In this study, we first determined the photoprotective effect of sanshool, a major component in Zanthoxylum bungeanum, on UVB-irradiated responses in human dermal fibroblasts (HDFs) and nude mouse. We found that sanshool treatment can protect cells against the effects of UVB irradiation by (i) increasing cell viability, (ii) inhibiting MMP expression, and (iii) inducing autophagy. We also used the recombinant CSF2 or anti-CSF2 antibody co-cultured with human dermal fibroblasts (HDFs) and found that CSF2 contributes to sanshool-induced autophagy. Sanshool hindered the UVB-induced activation of JAK2-STAT3 signaling in HDFs, thereby inhibiting the expression of MMPs and activation of autophagic flux. Exposure of the dorsal skin of hairless mice to UVB radiation and subsequent topical application of sanshool delayed the progression of skin inflammation, leading to autophagy and inhibiting the activation of JAK2-STAT3 signaling. These results provide a basis for the study of the photoprotective effect of sanshool and suggest that it can be potentially used as an agent against UVB-induced skin damage in humans.
Journal
Cell Death Dis
Publish Year
2019
Experiment Subject
human dermal fibroblasts (hdfs) and nude mouse
Experiment Type
Animal & Cell Experiment
Phenotype Related
Skin Cancer
Paper Title Cn
Paper Title En
Sanshool improves UVB-induced skin photodamage by targeting JAK2/STAT3-dependent autophagy
Bilingual Status
semi_complete