ReferenceID 6075
Puerarin suppresses hypoxia-induced vascular endothelial growth factor upregulation in human retinal pigmented epithelial cells by blocking JAK2/STAT3 pathway
Bioengineered
The purpose of this study was to explore the mechanism by which puerarin regulated the expression of hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in humans' retinal pigment epithelia
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Record Fields
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- Reference Id
- 6075
- Evidence Id
- 22665
- Core Evidence Id
- 22665
- Source Reference Id
- 5447
- Herb2 Reference Id
- HBREF006244
- Subject Paper Key
- HBIN041253_35510332
- Pubmed Id
- 35510332
- Doi
- 10.1080/21655979.2022.2070586
- Paper Title
- Puerarin suppresses hypoxia-induced vascular endothelial growth factor upregulation in human retinal pigmented epithelial cells by blocking JAK2/STAT3 pathway
- Paper Abstract
- The purpose of this study was to explore the mechanism by which puerarin regulated the expression of hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in humans' retinal pigment epithelial (RPE) cells under hypoxia. RPE cells (ARPE-19 and D407 cells) and a rat model of oxygen-induced retinopathy were used in the current study. Western blotting and ELISA were performed to detect the level of JAK2, phosphorylated JAK2, STAT3, phosphorylated STAT3, HIF-1α, and VEGF in cells. In addition, the interaction between JAK2 and STAT3 was determined using with a co-immunoprecipitation assay. We found puerarin inhibited hypoxia-induced upregulation of VEGF at both the mRNA and protein level via decreasing HIF-1α expression in RPE cells. Moreover, puerarin attenuated the interaction between JAK2 and STAT3, and subsequently blocking p-STAT3 nucleus translocation in vitro and in vivo . In conclusion, puerarin could effectively inhibit hypoxia-induced VEGF upregulation in RPE cells via mediated JAK2/STAT3 pathway.
- Journal
- Bioengineered
- Publish Year
- 2022
- Experiment Subject
- rat; human; d407 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Oxygen-induced Retinopathy
- Paper Title Cn
- Paper Title En
- Puerarin suppresses hypoxia-induced vascular endothelial growth factor upregulation in human retinal pigmented epithelial cells by blocking JAK2/STAT3 pathway
- Bilingual Status
- semi_complete