ReferenceID 6056

Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation

Phytother Res

Persistent chronic inflammation and fibrosis product accumulation aggravate tubulointerstitial fibrosis (TIF), leading to the progression of chronic kidney disease. The aim of this study was designed to investigate the e

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Reference Id
6056
Evidence Id
22646
Core Evidence Id
22646
Source Reference Id
5374
Herb2 Reference Id
HBREF006171
Subject Paper Key
HBIN040905_33118280
Pubmed Id
33118280
Doi
10.1002/ptr.6919
Paper Title
Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation
Paper Abstract
Persistent chronic inflammation and fibrosis product accumulation aggravate tubulointerstitial fibrosis (TIF), leading to the progression of chronic kidney disease. The aim of this study was designed to investigate the effect of protocatechualdehyde (PCA), a natural phenolic acid compound isolated from Salvia miltiorrhiza, on the unilateral ureteral obstruction (UUO)-induced fibrosis and inflammation and to elucidate the underlying mechanism in primary renal tubular epithelial cells (TECs). Results from the histology suggested that the moderate to severe deteriorations of renal dysfunction and the pathological changes in UUO could be relieved by PCA treatment. Mechanistic studies revealed that the effect of PCA was associated with the downregulation of Smad3 and NF-kappaB driven fibrosis and inflammation respectively. It is worth noting that PCA could inhibit the aberrant expression of inflammation cytokines such as iNOS, MCP-1, TNF-alpha in UUO, and IL-1beta-treated TECs. In addition, PCA also suppressed the expression of Smad3-dependent long noncoding RNA (lncRNA), 9884. Importantly, when overexpressing of lncRNA9884 in TECs by transfection of pcDNA3.1-lncRNA9884 plasmid, it revealed significant reversal of protein expression levels as that observed with only PCA, suggesting that PCA inhibits inflammation by mediating lncRNA9884/MCP-1 signaling pathway. Collectively, the current study establishes a foundational basis for PCA in future treatment of obstructive nephropathy.
Journal
Phytother Res
Publish Year
2021
Experiment Subject
uuo, and il-1beta-treated tecs
Experiment Type
Cell Experiment
Phenotype Related
Chronic Kidney Disease; Inflammation; Persistent Chronic Inflammation; Obstructive Nephropathy; Tubulointerstitial Fibrosis; Renal Dysfunction; Fibrosis; Unilateral Ureteral Obstruction
Paper Title Cn
Paper Title En
Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation
Bilingual Status
semi_complete