ReferenceID 6007
Piperine Targets Different Drug Resistance Mechanisms in Human Ovarian Cancer Cell Lines Leading to Increased Sensitivity to Cytotoxic Drugs
Int J Mol Sci
Our goal was to examine the anticancer effects of piperine against the resistant human ovarian cancer cells and to explore the molecular mechanisms responsible for its anticancer effects. Our study used drug-sensitive ov
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Record Fields
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- Reference Id
- 6007
- Evidence Id
- 22597
- Core Evidence Id
- 22597
- Source Reference Id
- 5278
- Herb2 Reference Id
- HBREF006075
- Subject Paper Key
- HBIN040071_33921897
- Pubmed Id
- 33921897
- Doi
- 10.3390/ijms22084243
- Paper Title
- Piperine Targets Different Drug Resistance Mechanisms in Human Ovarian Cancer Cell Lines Leading to Increased Sensitivity to Cytotoxic Drugs
- Paper Abstract
- Our goal was to examine the anticancer effects of piperine against the resistant human ovarian cancer cells and to explore the molecular mechanisms responsible for its anticancer effects. Our study used drug-sensitive ovarian cancer cell line W1 and its sublines resistant to paclitaxel (PAC) and topotecan (TOP). We analyzed the cytotoxic effect of piperine and cytostatic drugs using an MTT assay. The impact of piperine on protein expression was determined by immunofluorescence and Western blot. We also examined its effect on cell proliferation and migration. We noticed a different level of piperine resistance between cell lines. Piperine increases the cytotoxic effect of PAC and TOP in drug-resistant cells. We observed an increase in PTPRK expression correlated with decreased pTYR level after piperine treatment and downregulation of P-gp and BCRP expression. We also noted a decrease in COL3A1 and TGFBI expression in investigated cell lines and increased COL3A1 expression in media from W1PR2 cells. The expression of Ki67 protein and cell proliferation rate decreased after piperine treatment. Piperine markedly inhibited W1TR cell migration. Piperine can be considered a potential anticancer agent that can increase chemotherapy effectiveness in cancer patients.
- Journal
- Int J Mol Sci
- Publish Year
- 2021
- Experiment Subject
- human; patient; cell lines; drug-sensitive ovarian cancer cell line w1; w1pr2 cells; w1tr cell
- Experiment Type
- Cell Experiment
- Phenotype Related
- Ovarian Cancer; Drug-sensitive Ovarian Cancer; Cancer
- Paper Title Cn
- Paper Title En
- Piperine Targets Different Drug Resistance Mechanisms in Human Ovarian Cancer Cell Lines Leading to Increased Sensitivity to Cytotoxic Drugs
- Bilingual Status
- semi_complete