ReferenceID 5964

Perillyl alcohol reduces parasite sequestration and cerebrovascular dysfunction during experimental cerebral malaria

Antimicrob Agents Chemother

Cerebral malaria (CM) is a severe immunovasculopathy which presents high mortality rate (15-20%), despite the availability of artemisinin-based therapy. More effective immunomodulatory and/or antiparasitic therapies are

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Reference Id
5964
Evidence Id
22554
Core Evidence Id
22554
Source Reference Id
5190
Herb2 Reference Id
HBREF005987
Subject Paper Key
HBIN039240_33649109
Pubmed Id
33649109
Doi
10.1128/AAC.00004-21
Paper Title
Perillyl alcohol reduces parasite sequestration and cerebrovascular dysfunction during experimental cerebral malaria
Paper Abstract
Cerebral malaria (CM) is a severe immunovasculopathy which presents high mortality rate (15-20%), despite the availability of artemisinin-based therapy. More effective immunomodulatory and/or antiparasitic therapies are urgently needed. Experimental Cerebral Malaria (ECM) in mice is used to elucidate aspects involved in this pathology since manifests many of the neurological features of CM. In the present study, we evaluated the potential mechanisms involved in the protection afforded by perillyl alcohol (POH) in mouse strains susceptible to CM caused by Plasmodium berghei ANKA (PbA) infection through intranasal preventive treatment. Additionally, to evaluate the interaction of POH with the cerebral endothelium using an in vitro model of human brain endothelial cells (HBEC). Pharmacokinetic approaches demonstrated constant and prolonged levels of POH in the plasma and brain after a single intranasal dose. Treatment with POH effectively prevented vascular dysfunction. Furthermore, treatment with POH reduced the endothelial cell permeability and PbA s in the brain and spleen. Finally, POH treatment decreased the accumulation of macrophages and T and B cells in the spleen and downregulated the expression of endothelial adhesion molecules (ICAM-1, VCAM-1, and CD36) in the brain. POH is a potent monoterpene that prevents cerebrovascular dysfunction in vivo and in vitro, decreases parasite sequestration, and modulates different processes related to the activation, permeability, and integrity of the blood brain barrier (BBB), thereby preventing cerebral oedema and inflammatory infiltrates.
Journal
Antimicrob Agents Chemother
Publish Year
2021
Experiment Subject
mouse; human
Experiment Type
Animal Experiment
Phenotype Related
Vascular Dysfunction; Cerebral Malaria; Cerebrovascular Dysfunction; Inflammatory Infiltrates; Immunovasculopathy; Cerebral Oedema
Paper Title Cn
Paper Title En
Perillyl alcohol reduces parasite sequestration and cerebrovascular dysfunction during experimental cerebral malaria
Bilingual Status
semi_complete