ReferenceID 5937
Effect of paeoniflorin on acute lung injury induced by influenza A virus in mice. Evidences of its mechanism of action
Phytomedicine
BACKGROUND: Influenza often leads to acute lung injury (ALI). Few therapeutics options such as vaccines and other antiviral drugs are available. Paeoniflorin is a monoterpene glucoside isolated from the roots of Paeonia
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- Reference Id
- 5937
- Evidence Id
- 22527
- Core Evidence Id
- 22527
- Source Reference Id
- 5135
- Herb2 Reference Id
- HBREF005932
- Subject Paper Key
- HBIN038606_34509953
- Pubmed Id
- 34509953
- Doi
- 10.1016/j.phymed.2021.153724
- Paper Title
- Effect of paeoniflorin on acute lung injury induced by influenza A virus in mice. Evidences of its mechanism of action
- Paper Abstract
- BACKGROUND: Influenza often leads to acute lung injury (ALI). Few therapeutics options such as vaccines and other antiviral drugs are available. Paeoniflorin is a monoterpene glucoside isolated from the roots of Paeonia lactiflora Pall. that has showed good anti-inflammatory and anti-fibrotic effects. However, it is not known whether paeoniflorin has an effect on influenza virus-induced ALI. PURPOSE: To investigative the protective effect and potential mechanism of paeoniflorin on ALI induced by influenza A virus (IAV). STUDY DESIGN AND METHODS: The anti-influenza activity of paeoniflorin in vitro was investigated. Influenza virus A/FM/1/47 was intranasally infected in mice to induce ALI, and paeoniflorin (50 and 100 mg/kg) was given orally to mice during 5 days, beginning 2 h after infection. On day 6 post-infection, body and lung weights, histology and survival were observed, and the lungs were examined for viral load, cytokine and cellular pathway protein expression. RESULTS: Results showed that paeoniflorin (50 and 100 mg/kg) reduced IAV-induced ALI. It reduces pulmonary oedema and improves histopathological changes in the lung, and also diminishes the accumulation of inflammatory cells in the lung. It was shown that paeoniflorin (50 and 100 mg/kg) alleviated IAV-induced ALI, as evidenced by improved survival in infected mice (40% and 50%, respectively), reduced viral titer in lung tissue, improved histological changes, and reduced lung inflammation. Paeoniflorin also improves pulmonary fibrosis by reducing the levels of pulmonary fibrotic markers (collagen type IV, alpha-smooth muscle actin, hyaluronic acid, laminin, and procollagen type III) and downregulating the expression levels of type I collagen (Col I) and type III collagen (Col III) in the lung tissues. Additionally, paeoniflorin inhibits the expression of alphavbeta3, TGF-beta1, Smad2, NF-kappaB, and p38MAPK in the lung tissues. CONCLUSION: The results showed that paeoniflorin (50 and 100 mg/kg) protected against IAV-induced ALI, and the underlying mechanism may be related to the reduction of pro-inflammatory cytokine production and lung collagen deposition through down-regulation of activation of alphavbeta3/TGF-beta1 pathway in lung tissue.
- Journal
- Phytomedicine
- Publish Year
- 2021
- Experiment Subject
- mouse
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Pulmonary Oedema; Acute Lung Injury; Lung Inflammation; Influenza A Virus; Pulmonary Fibrosis
- Paper Title Cn
- Paper Title En
- Effect of paeoniflorin on acute lung injury induced by influenza A virus in mice. Evidences of its mechanism of action
- Bilingual Status
- semi_complete