ReferenceID 5920
Osthole enhances the bone mass of senile osteoporosis and stimulates the expression of osteoprotegerin by activating β-catenin signaling
Stem Cell Res Ther
INTRODUCTION: Osthole has a potential therapeutic application for anti-osteoporosis. The present study verified whether osthole downregulates osteoclastogenesis via targeting OPG. METHODS: In vivo, 12-month-old male mice
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Record Fields
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- Reference Id
- 5920
- Evidence Id
- 22510
- Core Evidence Id
- 22510
- Source Reference Id
- 5101
- Herb2 Reference Id
- HBREF005898
- Subject Paper Key
- HBIN038387_33640026
- Pubmed Id
- 33640026
- Doi
- 10.1186/s13287-021-02228-6
- Paper Title
- Osthole enhances the bone mass of senile osteoporosis and stimulates the expression of osteoprotegerin by activating β-catenin signaling
- Paper Abstract
- INTRODUCTION: Osthole has a potential therapeutic application for anti-osteoporosis. The present study verified whether osthole downregulates osteoclastogenesis via targeting OPG. METHODS: In vivo, 12-month-old male mice were utilized to evaluate the effect of osthole on bone mass. In vitro, bone marrow stem cells (BMSCs) were isolated and extracted from 3-month-old OPG-/- mice and the littermates of OPG+/+ mice. Calvaria osteoblasts were extracted from 3-day-old C57BL/6J mice or 3-day-old OPG-/- mice and the littermates of OPG+/+ mice. RESULTS: Osthole significantly increased the gene and protein levels of OPG in primary BMSCs in a dose-dependent manner. The deletion of the OPG gene did not affect beta-catenin expression. The deletion of the beta-catenin gene inhibited OPG expression in BMSCs, indicating that osthole stimulates the expression of OPG via activation of beta-catenin signaling. CONCLUSION: Osthole attenuates osteoclast formation by stimulating the activation of beta-catenin-OPG signaling and could be a potential drug for the senile osteoporosis.
- Journal
- Stem Cell Res Ther
- Publish Year
- 2021
- Experiment Subject
- mouse
- Experiment Type
- Animal Experiment
- Phenotype Related
- Senile Osteoporosis; Anti-osteoporosis
- Paper Title Cn
- Paper Title En
- Osthole enhances the bone mass of senile osteoporosis and stimulates the expression of osteoprotegerin by activating β-catenin signaling
- Bilingual Status
- semi_complete