ReferenceID 5902
Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation
Oxid Med Cell Longev
Ophiopogonin D (OPD), a compound from the Chinese herb Radix Ophiopogonis, reportedly induces increased levels of cytochrome P450 2J3 (CYP2J3)/epoxyeicosatrienoic acids (EETs) and Ca2+ in rat cardiomyocytes. Little is kn
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Record Fields
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- Reference Id
- 5902
- Evidence Id
- 22492
- Core Evidence Id
- 22492
- Source Reference Id
- 5063
- Herb2 Reference Id
- HBREF005860
- Subject Paper Key
- HBIN038205_33488937
- Pubmed Id
- 33488937
- Doi
- 10.1155/2020/8857906
- Paper Title
- Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation
- Paper Abstract
- Ophiopogonin D (OPD), a compound from the Chinese herb Radix Ophiopogonis, reportedly induces increased levels of cytochrome P450 2J3 (CYP2J3)/epoxyeicosatrienoic acids (EETs) and Ca2+ in rat cardiomyocytes. Little is known regarding the specific mechanism between CYP2J3 and Ca2+ homeostasis. Here, we investigated whether CYP2J3 is involved in the protective action of OPD on the myocardium by activating the Ca2+ homeostasis-related protein complex (SERCA2a and PLB) in H9c2 rat cardiomyoblast cells. The interaction between SERCA2a and PLB was measured using fluorescence resonance energy transfer. OPD attenuated heart failure and catalyzed the active transport of Ca2+ into the sarcoplasmic reticulum by inducing the phosphorylation of PLB and promoting the SERCA2a activity. These beneficial effects of OPD on heart failure were abolished after knockdown of CYP2J3 in a model of heart failure. Together, our results identify CYP2J3 as a critical intracellular target for OPD and unravel a mechanism of CYP2J3-dependent regulation of intracellular Ca2+.
- Journal
- Oxid Med Cell Longev
- Publish Year
- 2020
- Experiment Subject
- rat; h9c2 rat cardiomyoblast cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Heart Failure
- Paper Title Cn
- Paper Title En
- Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation
- Bilingual Status
- semi_complete