ReferenceID 5854
Effect of Neferine on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice
Int J Mol Sci
Atopic dermatitis (AD) is a chronic and persistent inflammatory skin disease characterized by eczematous lesions and itching, and it has become a serious health problem. However, the common clinical treatments provide li
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- Reference Id
- 5854
- Evidence Id
- 22444
- Core Evidence Id
- 22444
- Source Reference Id
- 4962
- Herb2 Reference Id
- HBREF005759
- Subject Paper Key
- HBIN036518_34361003
- Pubmed Id
- 34361003
- Doi
- 10.3390/ijms22158237
- Paper Title
- Effect of Neferine on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice
- Paper Abstract
- Atopic dermatitis (AD) is a chronic and persistent inflammatory skin disease characterized by eczematous lesions and itching, and it has become a serious health problem. However, the common clinical treatments provide limited relief and are accompanied by adverse effects. Therefore, there is a need to develop novel and effective therapies to treat AD. Neferine is a small molecule compound isolated from the green embryo of the mature seeds of lotus (Nelumbo nucifera). It has a bisbenzylisoquinoline alkaloid structure. Relevant studies have shown that neferine has many pharmacological and biological activities, including anti-inflammatory, anti-thrombotic, and anti-diabetic activities. However, there are very few studies on neferine in the skin, especially the related effects on inflammatory skin diseases. In this study, we proved that it has the potential to be used in the treatment of atopic dermatitis. Through in vitro studies, we found that neferine inhibited the expression of cytokines and chemokines in TNF-alpha/IFN-gamma-stimulated human keratinocyte (HaCaT) cells, and it reduced the phosphorylation of MAPK and the NF-kappaB signaling pathway. Through in vivo experiments, we used 2,4-dinitrochlorobenzene (DNCB) to induce atopic dermatitis-like skin inflammation in a mouse model. Our results show that neferine significantly decreased the skin barrier damage, scratching responses, and epidermal hyperplasia induced by DNCB. It significantly decreased transepidermal water loss (TEWL), erythema, blood flow, and ear thickness and increased surface skin hydration. Moreover, it also inhibited the expression of cytokines and the activation of signaling pathways. These results indicate that neferine has good potential as an alternative medicine for the treatment of atopic dermatitis or other skin-related inflammatory diseases.
- Journal
- Int J Mol Sci
- Publish Year
- 2021
- Experiment Subject
- mouse; human; tnf-alpha/ifn-gamma-stimulated human keratinocyte (hacat) cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Atopic Dermatitis; Eczematous Lesions; Inflammatory Skin Diseases; Erythema; Chronic And Persistent Inflammatory Skin Disease; Epidermal Hyperplasia; Skin-related Inflammatory Diseases; Atopic Dermatitis-like Skin Inflammation; Itching
- Paper Title Cn
- Paper Title En
- Effect of Neferine on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice
- Bilingual Status
- semi_complete