ReferenceID 5852
Anti-Adipogenic Effect of Neferine in 3T3-L1 Cells and Primary White Adipocytes
Nutrients
Neferine, an alkaloid component extracted from lotus seed embryos, is known for its anti-inflammatory, anticancer, and antioxidant properties. However, the anti-adipogenic activity of neferine has not been thoroughly inv
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Record Fields
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- Reference Id
- 5852
- Evidence Id
- 22442
- Core Evidence Id
- 22442
- Source Reference Id
- 4959
- Herb2 Reference Id
- HBREF005756
- Subject Paper Key
- HBIN036518_32580414
- Pubmed Id
- 32580414
- Doi
- 10.3390/nu12061858
- Paper Title
- Anti-Adipogenic Effect of Neferine in 3T3-L1 Cells and Primary White Adipocytes
- Paper Abstract
- Neferine, an alkaloid component extracted from lotus seed embryos, is known for its anti-inflammatory, anticancer, and antioxidant properties. However, the anti-adipogenic activity of neferine has not been thoroughly investigated. In this study, neferine was found to inhibit lipid accumulation in a dose-dependent manner during the differentiation of 3T3-L1 cells without inducing cytotoxicity. Real-time polymerase chain reaction and immunoblot analysis revealed the downregulation in the expression of peroxisome proliferator activated receptor gamma (PPARgamma), CCAAT/enhancer-binding protein alpha (C/EBPalpha), sterol regulatory element-binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) and the upregulation in carnitine palmitoyltransferase-1 (CPT-1) and sirtuin 1 (SIRT1) levels following neferine treatment. Furthermore, neferine increased the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), which is an important regulator of fatty acid oxidation. Our result indicates that neferine attenuates adipogenesis and promotes lipid metabolism by activating AMPK-mediated signaling. Therefore, neferine may serve as a therapeutic candidate for obesity treatment.
- Journal
- Nutrients
- Publish Year
- 2020
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Obesity
- Paper Title Cn
- Paper Title En
- Anti-Adipogenic Effect of Neferine in 3T3-L1 Cells and Primary White Adipocytes
- Bilingual Status
- semi_complete