ReferenceID 5741
Melatonin and doxorubicin synergistically enhance apoptosis via autophagy-dependent reduction of AMPKα1 transcription in human breast cancer cells
Exp Mol Med
Doxorubicin is one of the most effective agents used to treat various cancers, including breast cancer, but its usage is limited by the risk of adverse effects, including cardiotoxicity. Melatonin, a natural hormone that
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 5741
- Evidence Id
- 22331
- Core Evidence Id
- 22331
- Source Reference Id
- 4720
- Herb2 Reference Id
- HBREF005517
- Subject Paper Key
- HBIN034657_34584194
- Pubmed Id
- 34584194
- Doi
- 10.1038/s12276-021-00675-y
- Paper Title
- Melatonin and doxorubicin synergistically enhance apoptosis via autophagy-dependent reduction of AMPKα1 transcription in human breast cancer cells
- Paper Abstract
- Doxorubicin is one of the most effective agents used to treat various cancers, including breast cancer, but its usage is limited by the risk of adverse effects, including cardiotoxicity. Melatonin, a natural hormone that functions as a major regulator of circadian rhythms, has been considered a supplemental component for doxorubicin due to its potential to improve its effectiveness. However, the mechanisms and biological targets of the combination of melatonin and doxorubicin with respect to cancer cell death are not well understood. In the present study, we found that melatonin synergized with doxorubicin to induce apoptosis of breast cancer cells by decreasing the expression of AMP-activated protein kinase alpha1 (AMPK alpha1), which acts as a critical survival factor for cancer cells. This cotreatment-induced reduction in AMPKalpha1 expression occurred at the transcriptional level via an autophagy-dependent mechanism. The synergistic effects of the combined treatment were evident in many other cancer cell lines, and melatonin was also highly effective in inducing cancer death when combined with other cancer drugs, including cisplatin, 5-fluorouracil, irinotecan, and sorafenib. AMPKalpha1 expression was decreased in all of these cases, suggesting that reducing AMPKalpha1 can be considered an effective method to increase the sensitivity of cancer cells to doxorubicin treatment.
- Journal
- Exp Mol Med
- Publish Year
- 2021
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cardiotoxicity; Cancers; Cancer; Breast Cancer
- Paper Title Cn
- Paper Title En
- Melatonin and doxorubicin synergistically enhance apoptosis via autophagy-dependent reduction of AMPKα1 transcription in human breast cancer cells
- Bilingual Status
- semi_complete