ReferenceID 5685
Matrine Inhibits CNS Autoimmunity Through an IFN-β-Dependent Mechanism
Front Immunol
Matrine (MAT), a quinolizidine alkaloid component derived from the root of Sophora flavescens, suppresses experimental autoimmune encephalomyelitis (EAE), the animal model of multiple sclerosis (MS), by inducing the prod
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Record Fields
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- Reference Id
- 5685
- Evidence Id
- 22275
- Core Evidence Id
- 22275
- Source Reference Id
- 4612
- Herb2 Reference Id
- HBREF005409
- Subject Paper Key
- HBIN034558_33101289
- Pubmed Id
- 33101289
- Doi
- 10.3389/fimmu.2020.569530
- Paper Title
- Matrine Inhibits CNS Autoimmunity Through an IFN-β-Dependent Mechanism
- Paper Abstract
- Matrine (MAT), a quinolizidine alkaloid component derived from the root of Sophora flavescens, suppresses experimental autoimmune encephalomyelitis (EAE), the animal model of multiple sclerosis (MS), by inducing the production of immunomodulatory molecules, e.g., IL-10. In an effort to find the upstream pathway(s) of the mechanism underlying these effects, we have tested certain upregulated immunomodulatory molecules. Among them, we found increased levels of IL-27 and IFN-beta, one of the first-line MS therapies. Indeed, while low levels of IFN-beta production in sera and type I interferon receptor (IFNAR1) expression in spinal cord of saline-treated control EAE mice were detected, they were significantly increased after MAT treatment. Increased numbers of CD11b+IFN-beta+ microglia/infiltrating macrophages were observed in the CNS of MAT-treated mice. The key role of IFN-beta induction in the suppressive effect of MAT on EAE was further verified by administration of anti-IFN-beta neutralizing antibody, which largely reversed the therapeutic effect of MAT. Further, we found that, while MAT treatment induced production of IL-27 and IL-10 by CNS microglia/macrophages, this effect was significantly reduced by IFN-beta neutralizing antibody. Finally, the role of IFN-beta in MAT-induced IL-27 and IL-10 production was further confirmed in human monocytes in vitro. Together, our study demonstrates that MAT exerts its therapeutic effect in EAE through an IFN-beta/IL-27/IL-10 pathway, and is likely a novel, safe, low-cost, and effective therapy as an alternative to exogenous IFN-beta for MS.
- Journal
- Front Immunol
- Publish Year
- 2020
- Experiment Subject
- mouse; human
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Multiple Sclerosis; Autoimmune Encephalomyelitis
- Paper Title Cn
- Paper Title En
- Matrine Inhibits CNS Autoimmunity Through an IFN-β-Dependent Mechanism
- Bilingual Status
- semi_complete