ReferenceID 5674
Mangiferin induces the expression of a thermogenic signature via AMPK signaling during brown-adipocyte differentiation
Food Chem Toxicol
Mangiferin (MF) from Mangifera indica has been serendipitously found to ameliorate obesity and is used as an antioxidant, anti-inflammatory, antimicrobial, and anticancer agent. Nonetheless, the mechanism of MF-induced b
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Record Fields
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- Reference Id
- 5674
- Evidence Id
- 22264
- Core Evidence Id
- 22264
- Source Reference Id
- 4585
- Herb2 Reference Id
- HBREF005382
- Subject Paper Key
- HBIN034394_32417366
- Pubmed Id
- 32417366
- Doi
- 10.1016/j.fct.2020.111415
- Paper Title
- Mangiferin induces the expression of a thermogenic signature via AMPK signaling during brown-adipocyte differentiation
- Paper Abstract
- Mangiferin (MF) from Mangifera indica has been serendipitously found to ameliorate obesity and is used as an antioxidant, anti-inflammatory, antimicrobial, and anticancer agent. Nonetheless, the mechanism of MF-induced brown-adipose-tissue activation has not been studied. Therefore, we investigated the effect of MF on thermogenic features during brown-adipocyte differentiation. Treatment with MF improved the expression of a brown-fat signature and of mitochondrial-mass-related genes, thus resulting in UCP1 induction. MF also raised the expression of other thermogenic regulators, including peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1alpha), PR domain-containing protein 16 (PRDM16), and peroxisome proliferator-activated receptors alpha and gamma (PPAR-alpha and -gamma). MF promoted mitochondrial biogenesis, judging by increased expression of cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA), mitochondrial transcription factor A (TFAM), iodothyronine deiodinase 2 (DIO2), cytochrome c oxidase subunit 7A (COX7A), cyclooxygenase 2 (COX2), sirtuin 1 (SIRT1), and nuclear respiratory factor 1 (NRF1). MF treatment increased the mitochondrial DNA amount and improved mitochondrial respiratory function by increasing the oxygen consumption rate during brown-adipocyte differentiation. A gene knockdown assay involving small interfering RNA and competitive inhibition with dorsomorphin revealed that MF may promote thermogenesis in brown preadipocytes via activation of AMPK signaling. Collectively, our findings suggest that MF may be a novel pharmaceutical agent that can ameliorate obesity via activation of brown adipose tissue.
- Journal
- Food Chem Toxicol
- Publish Year
- 2020
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Obesity
- Paper Title Cn
- Paper Title En
- Mangiferin induces the expression of a thermogenic signature via AMPK signaling during brown-adipocyte differentiation
- Bilingual Status
- semi_complete