ReferenceID 5653

Lycopene intake induces colonic regulatory T cells in mice and suppresses food allergy symptoms

Pediatr Allergy Immunol

BACKGROUND: Food allergy (FA) is a common disease in children; thus, a high level of safety is required for its prevention and treatment. Colonic regulatory T cells (Tregs) have been suggested to attenuate FA. We investi

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Reference Id
5653
Evidence Id
22243
Core Evidence Id
22243
Source Reference Id
4536
Herb2 Reference Id
HBREF005333
Subject Paper Key
HBIN033972_34716962
Pubmed Id
34716962
Doi
10.1111/pai.13691
Paper Title
Lycopene intake induces colonic regulatory T cells in mice and suppresses food allergy symptoms
Paper Abstract
BACKGROUND: Food allergy (FA) is a common disease in children; thus, a high level of safety is required for its prevention and treatment. Colonic regulatory T cells (Tregs) have been suggested to attenuate FA. We investigated the Treg-inducing ability and anti-FA effects of carotenoids, a pigment contained in vegetables and fruits. METHODS: C57BL/6N mice were fed a diet containing 0.01% (w/w) of lycopene, beta-carotene, astaxanthin or lutein for 4 weeks, and the population of colonic Tregs was assessed. Subsequently, to evaluate the Treg-inducing ability of lycopene, splenic naive CD4+ T cells from BALB/c mice were cultured with anti-CD3/CD28 antibody, TGF-beta and lycopene, and the frequencies of Tregs were examined. The effect of 0.1% (w/w) lycopene containing diet on FA was investigated in OVA-induced FA model BALB/c mice. RESULTS: In screening, only lycopene significantly increased the frequency and number of colonic Tregs. Lycopene also increased Treg differentiation in splenic naive CD4+ T cells. In FA mice, lycopene feeding significantly increased the number of colonic Tregs and attenuated allergic symptoms. The expression levels of IL-4, IL-9 and IL-13 mRNA in colonic mucosa were also significantly reduced by lycopene. IL-9 is known to induce proliferation of mast cells, and we found that lycopene feeding significantly reduced the number of mast cells in the colonic mucosa of FA mice. CONCLUSION: Our results suggest that lycopene, a carotenoid present in many common foods on the market, may have the potential to induce colonic Tregs and suppress FA symptoms.
Journal
Pediatr Allergy Immunol
Publish Year
2022
Experiment Subject
mouse; children
Experiment Type
Animal Experiment
Phenotype Related
Food Allergy
Paper Title Cn
Paper Title En
Lycopene intake induces colonic regulatory T cells in mice and suppresses food allergy symptoms
Bilingual Status
semi_complete