ReferenceID 5649
Lycopene Protects against Smoking-Induced Lung Cancer by Inducing Base Excision Repair
Antioxidants (Basel)
BACKGROUND: Oxidative stress plays a critical role in lung cancer progression. Carotenoids are efficient antioxidants. The objective of this study was to explore the efficacy of all-trans retinoic acid (ATRA) and caroten
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Record Fields
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- Reference Id
- 5649
- Evidence Id
- 22239
- Core Evidence Id
- 22239
- Source Reference Id
- 4527
- Herb2 Reference Id
- HBREF005324
- Subject Paper Key
- HBIN033972_32708354
- Pubmed Id
- 32708354
- Doi
- 10.3390/antiox9070643
- Paper Title
- Lycopene Protects against Smoking-Induced Lung Cancer by Inducing Base Excision Repair
- Paper Abstract
- BACKGROUND: Oxidative stress plays a critical role in lung cancer progression. Carotenoids are efficient antioxidants. The objective of this study was to explore the efficacy of all-trans retinoic acid (ATRA) and carotenoids in cigarette smoke-induced oxidative stress within A549 human lung cancer epithelial cells. METHODS: A549 cells were pretreated with 1-nM, 10-nM, 100-nM, 1-muM and 10-muM ATRA, beta-carotene (BC) and lycopene for 24 h, followed by exposure to cigarette smoke using a smoking chamber. RESULTS: The OxyBlot analysis showed that smoking significantly increased oxidative stress, which was inhibited by lycopene at 1 nM and 10 nM (p < 0.05). In the cells exposed to smoke, lycopene increased 8-oxoguanine DNA glycosylase (OGG1) expression at 1 nM, 10 nM, 100 nM, and 1 muM (p < 0.05), but not at 10 muM. Lycopene at lower doses also improved Nei like DNA glycosylases (NEIL1, NEIL2, NEIL3), and connexin-43 (Cx43) protein levels (p < 0.05). Interestingly, lycopene at lower concentrations promoted OGG1 expression within the cells exposed to smoke to an even greater extent than the cells not exposed to smoke (p < 0.01). This may be attributed to the increased SR-B1 mRNA levels with cigarette smoke exposure (p < 0.05). CONCLUSIONS: Lycopene treatment at a lower dosage could inhibit smoke-induced oxidative stress and promote genome stability. These novel findings will shed light on the molecular mechanism of lycopene action against lung cancer.
- Journal
- Antioxidants (Basel)
- Publish Year
- 2020
- Experiment Subject
- human; a549 cells; a549 human lung cancer epithelial cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Lung Cancer
- Paper Title Cn
- Paper Title En
- Lycopene Protects against Smoking-Induced Lung Cancer by Inducing Base Excision Repair
- Bilingual Status
- semi_complete