ReferenceID 5603
Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury
Cell Calcium
TRPV1 and TRPA1 are cation channels that play key roles in inflammatory signaling pathways. They are co-expressed on airway C-fibers, where they exert synergistic effects on causing inflammation and cough. Licorice, the
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 5603
- Evidence Id
- 22193
- Core Evidence Id
- 22193
- Source Reference Id
- 4439
- Herb2 Reference Id
- HBREF005236
- Subject Paper Key
- HBIN033383_32388008
- Pubmed Id
- 32388008
- Doi
- 10.1016/j.ceca.2020.102198
- Paper Title
- Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury
- Paper Abstract
- TRPV1 and TRPA1 are cation channels that play key roles in inflammatory signaling pathways. They are co-expressed on airway C-fibers, where they exert synergistic effects on causing inflammation and cough. Licorice, the root of Glycyrrhiza uralensis, has been widely used in China as an anti-inflammatory and anti-coughing herb. To learn if TRPV1 and TRPA1 might be key targets of the anti-inflammatory and antitussive effects of licorice, we examined liquiritin, the main flavonoid compound and active ingredient of licorice, on agonist-evoked TRPV1 and TRPA1 activation. Liquiritin inhibited capsaicin- and allyl isothiocyanate-evoked TRPV1 and TRPA1 whole-cell currents, respectively, with a similar potency and maximal inhibition. In a mouse acute lung injury (ALI) model induced by the bacterial endotoxin lipopolysaccharide, which involves both TRPV1 and TRPA1, an oral gavage of liquiritin prevented tissue damage and suppressed inflammation and the activation of NF-kappaB signaling pathway in the lung tissue. Liquiritin also suppressed LPS-induced increase in TRPV1 and TRPA1 protein expression in the lung tissue, as well as TRPV1 and TRPA1 mRNA levels in cells contained in mouse bronchoalveolar lavage fluid. In cultured THP-1 monocytes, liguiritin, or TRPV1 and TRPA1 antagonists capsazepine and HC030031, respectively, diminished not only cytokine-induced upregulation of NF-kappaB function but also TRPV1 and TRPA1 expression at both protein and mRNA levels. We conclude that the anti-inflammatory and antitussive effects of liquiritin are mediated by the dual inhibition of TRPV1 and TRPA1 channels, which are upregulated in nonneuronal cells through the NF-kappaB pathway during airway inflammation via a positive feedback mechanism.
- Journal
- Cell Calcium
- Publish Year
- 2020
- Experiment Subject
- mouse; cultured thp-1 monocytes
- Experiment Type
- Animal Experiment
- Phenotype Related
- Inflammation; Cough; Acute Lung Injury; Airway Inflammation
- Paper Title Cn
- Paper Title En
- Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury
- Bilingual Status
- semi_complete